Summary
PAH-clearance studies were carried out separately on each kidney of both normal rats and rats with experimental Goldblatt-Hypertension. The rat and especially the rat kidney metabolizes a certain proportion of PAH-molecules 1. by splitting off the glycine-group forming p-aminobenzoate (PAB); 2. both PAH and PAB undergo partial N-acetylation. N-acetylated metabolites cannot be detected by the conventional analysis of PAH. Resorption of benzoates in the kidney results in a relatively high benzoate concentration in the renal vein. The complications created by these facts are discussed in relation to RPF-estimation using PAH. A correct determination of RPF by PAH is possible only if the analysis is carried out after an acid hydrolysis to make the detection of N-acetylated metabolites possible and if renal venous blood samples can be collected to correct for the reabsorbed benzoates. The clamped kidney of rats with Goldblatt-Hypertension is able to split glycine off from PAH but its capacity to N-acetylate is reduced. As a result identical PAH-clearances are found in both kidneys of these animals although their true RPF differ significantly.
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Mit Unterstützung der Deutschen Forschungsgemeinschaft im Rahmen des SFB 89 — Kardiologie Göttingen.
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Mályusz, M., Girndt, J., Mályusz, G. et al. Die Metabolisierung von p-Aminohippurat in Nieren von normalen Ratten und Ratten mit experimentellem Goldblatt-Hochdruck. Pflügers Arch. 333, 156–165 (1972). https://doi.org/10.1007/BF00586914
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DOI: https://doi.org/10.1007/BF00586914