Abstract
The oxygenation capabilities of a new generation three phase – two region 1200 l bioreactor employed for the cultivation of anchorage dependent animal cells were investigated experimentally. A mathematical model has also been developed that explains qualitatively the observed oxygenation characteristics. This type of bioreactor, that uses microcarrier support particles, has two distinct mesh-separated regions so that the air bubbles in the oxygenation region do not come into contact with the microcarriers in the cell (bubble-free) region. Implications on achievable maximum cell densities are also discussed.
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Received: 4 September 1996
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Kalogerakis, N., Behie, L. Oxygenation capabilities of basket-type bioreactors for microcarrier cultures of anchorage-dependent cells. Bioprocess Engineering 17, 151–156 (1997). https://doi.org/10.1007/s004490050369
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DOI: https://doi.org/10.1007/s004490050369