Abstract
Penicillin acylase obtained from E. Coli (E. C. 3.5.1.11) was covalently bound via glutaric aldehyde to acrylic carriers crosslinked with divinylbenzene or ethylene glycol dimethacrylate. The best enzymatic preparation was obtained by using ethyl acrylate/ ethylene glycol dimethacrylate copolymer. 1 cm3 of the carrier bound 6.4 mg of protein, having 72% activity in relation to the native enzyme. The preparation lost only 10% of its initial activity after 100 d of storage at 4°C. A negligible effect of immobilization on the enzyme activity at different temperatures or pH as well as significant increase of the stability of the immobilized enzyme at elevated temperatures were observed.
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Abbreviations
- BA:
-
butyl acrylate
- AE:
-
ethyl acrylate
- PA:
-
penicillin acylase
- 6-APA:
-
6-aminopenicillanic acid
- EGDMA:
-
ethylene glycol dimethacrylate
- DVB:
-
divinylbenzene
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Bryjak, J., Trochimczuk, A. & Noworyta, A. Immobilization of penicillin acylase on acrylic carriers. Bioprocess Eng. 4, 159–162 (1989). https://doi.org/10.1007/BF00369394
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DOI: https://doi.org/10.1007/BF00369394