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Enhanced prolylhydroxylase activity in the posterior annulus fibrosus of canine intervertebral discs following long-term running exercise

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Résumé

Cette étude porte sur l'effet d'un entraînement au long cours sur la concentration discale en collagène (hydroxyproline), en enzymes intervenant dans la synthèse du collagène (prolyl-4-hydroxylase, PH et galactosyl-hydroxylysyl-glucosyltransférase, GGT) et en liaisons hydroxypyrinidium (Pyr) chez dix beagle femelles. Ces chiens ont été entraînés à la course pendant une année, à partir de l'âge de 15 semaines. La distance quotidiennement parcourue a été progressivement augmentée jusqu'à 40 km par jour durant les 15 dernières semaines. Dix chiens non entraînés et provenant du même élevage ont servi de témoins. La zone centrale des disques et les moitiés antérieure et postérieure de l'annulus provenant des disques C2-C3, T10–T11, T11–T12, et L4–L5 ont été soumises à une analyse biochimique. Les liaisons chimiques ont été étudiées au niveau des constituants de la partie antérieure de l'annulus du disque T10–T11. Les concentrations en hydroxyproline et en Pyr sont restées semblables dans les deux groupes. Sous l'action de la course, la PH et la GGT sont apparues significativement élevées dans la partie postérieure des anneaux fibreux des disques thoraciques et lombaires et dans les zones centrales des disques lombaires. Dans les zones centrales des disques thoraciques, la GGT était significativement réduite. Ces résultats laissent supposer que l'élévation du métabolisme du collagène dans la partie postérieure des anneaux fibreux thoraciques et lombaires résulte de l'augmentation locale du surmenage rachidien.

Summary

The effect of long-term exercise on the intervertebral disc collagen concentration (hydroxyproline), collagen-synthesizing enzymes (prolyl-4-hydroxylase, PH, and galactosyl-hydroxylysyl glucosyltransferase, GGT) and hydroxypyridinium crosslinks was studied in ten female beagle dogs. The dogs were run on a treadmill for 1 year starting at the age of 15 weeks. The daily running distance was gradually increased to 40 km, which distance the dogs ran for the final 15 weeks. Ten untrained dogs from the same breeding colony served as controls. The nucleus pulposus and anterior and posterior halves of the annulus fibrosus of C2–3, T10–12, L4–5 disc segments were analysed. Crosslinks were measured from the anterior annulus fibrosus of the T10–11 disc. Hydroxyproline and hydroxypyridinium concentrations remained similar in both groups. PH and GGT were significantly elevated by running in the posterior annulus fibrosus of the thoracic and lumbar discs and in the lumbar nucleus pulposus. In the thoracic nucleus pulposus GGT was reduced significantly. The results suggest activated collagen metabolism in the posterior annulus fibrosus of the thoracic and lumbar discs as a result of locally increased strains on the spine.

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Authors and Affiliations

  1. Department of Anatomy, University of Kuopio, Kuopio, Finland

    K. Puustjärvi, M. Tammi & H. J. Helminen

  2. Sports Clinic, Deaconess Institute of Oulu, Oulu, Finland

    T. Takala & W. Wang

  3. Department of Health Sciences, University of Jyväskylä, Jyväskylä, Finland

    V. Kovanen

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  1. K. Puustjärvi
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  2. T. Takala
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  4. M. Tammi
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  5. H. J. Helminen
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  6. V. Kovanen
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The research was carried out at the Department of Anatomy, University of Kuopio

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Puustjärvi, K., Takala, T., Wang, W. et al. Enhanced prolylhydroxylase activity in the posterior annulus fibrosus of canine intervertebral discs following long-term running exercise. Eur Spine J 2, 126–131 (1993). https://doi.org/10.1007/BF00301408

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