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Optical rotatory dispersion in quantitative analysis: Microdetermination of some steroid sex-hormones

Optische Rotationsdispersion in der quantitativen Analyse: Mikrobestimmung einiger Steroid-Sexualhormone

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Zusammenfassung

Die Rotationsdispersionsspektren von Testosteron, Progesteron, Oestron und Androstanalon weisen Rotations-maxima bei 250, 300, 310 und 305 nm auf. Die Rotationswerte sind der Hormonkonzentration von 15 μg bis 2,5 mg/ml linear proportional. Binäre Gemische dieser Hormone können durch Rotationsmessung bei zwei Wellenlängen simultan bestimmt werden. Durchschnittlich werden 99,8% wiedergefunden, der maximale Fehler beträgt ± 2% absolut.

Summary

A method is described for microdetermination of some steroid sex-hormones based on the optical properties of these compounds. The optical rotatory dispersion spectra of testosterone, progesterone, estrone and androstanalone show rotation maxima at 250, 300, 310 and 305 nm, respectively. The rotation values are proportional, in linear relations, to the hormone concentration ranging from 15 μg up to 2.5 mg/ml. Binary mixtures of some of these hormones are also simultaneously determined by rotation measurement at two wave lengths. The results show an average recovery of 99.8%, the maximum absolute error being ± 2%.

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Authors and Affiliations

  1. Research Microanalytical Laboratory, Department of Chemistry, Faculty of Science, Ain Shams University, Cairo, Egypt

    Saad S. M. Hassan

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  1. Saad S. M. Hassan
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Hassan, S.S.M. Optical rotatory dispersion in quantitative analysis: Microdetermination of some steroid sex-hormones. Z. Anal. Chem. 269, 363–367 (1974). https://doi.org/10.1007/BF00430719

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  • DOI: https://doi.org/10.1007/BF00430719

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