Summary
A study of fluorescence properties of Cloxazolam and Oxazolam has been carried out. pKa-values were calculated and a spectrofluorimetric method was developed in acidic hydroalcoholic medium for the determination of the drugs. The fluorescence intensity was linear with the concentration up to at least 6.00×10−6 mol/l for Cloxazolam and 5.17×10−6 mol/l for Oxazolam. Detection limits obtained were 2.91×10−8 mol/l and 4.56×10−8 mol/l for Cloxazolam and Oxazolam, respectively. Spectrofluorimetric methods were applied to the determination of both drugs in pharmaceuticals and errors lower than 2.5% were obtained. Spectrophotometric determination methods were also developed.
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Melón, A., Alonso, R.M. & Jiménez, R.M. Spectrofluorimetric determination of benzodiazepinooxazoles. Fresenius J Anal Chem 337, 403–407 (1990). https://doi.org/10.1007/BF00322220
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DOI: https://doi.org/10.1007/BF00322220