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Determination of l-lactate by differential pulse polarography with covalently immobilized enzyme on Chitopearl

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Summary

The covalent bonding of l-lactate oxidase (no E.C.) to cross-linked copolymers of chitosan (Chitopearl) using the glutaraldehyde method is described and applied to the determination of l-lactate in serums of diseased and normal animals. The bioreactor packing of the immobilized enzyme is stable for at least 4 months and can be continuously used for the rapid and simple determination of l-lactate. The reduction current of pyruvate, corresponding to the product of enzymatic reaction, is determined by differential pulse polarography. The reproducibility (RSD) for 10 μmol/l l-lactate is 0.78% (n=6) and the detection limit is 0.62 μmol/l (k=2, confidence level 97.72%).

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Hokkaido University, 060, Sapporo, Japan

    Kiyoshi Hasebe, Satoshi Hikima & Hitoshi Yoshida

Authors
  1. Kiyoshi Hasebe
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  2. Satoshi Hikima
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  3. Hitoshi Yoshida
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Hasebe, K., Hikima, S. & Yoshida, H. Determination of l-lactate by differential pulse polarography with covalently immobilized enzyme on Chitopearl. Fresenius J Anal Chem 339, 261–263 (1991). https://doi.org/10.1007/BF00325750

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  • DOI: https://doi.org/10.1007/BF00325750

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