Summary
The activities of acid orthophosphoric-monoester-phosphohydrolases in the supra-opticus nucleus and the neurohypophysis of the rat were measured at pH 4.1 and 5.3 by biochemical assay and by histospectrophotometric method after a thirsting period of 1–12 days. For the neurohypophysis the biochemical and histophotometrical results are convergent. These enzymatic activities increase during the entire experimental period but if the biochemical results are expressed for 100 μg of proteins the elevation is only significant at pH 4.1. For the supra-optic nucleus, changes in enzymatic activities are different by histophotometric and biochemical methods. It is assumed that in the rat hypothalamo-neurohypophyseal system the acid phosphatases are distributed between two separate pools: (1) one strongly bound to cytoplasmic membrane structures, particulary abundant in the perikarya of the supra-optic nucleus, and (2) another pool of “free” or easily discharged enzyme, the major component in the neurohypophysis; our biochemical assay measures only this “free pool”. The role of these pools in the synthesis and release of vasopressin is presumably different.
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Aerts, C., Guillaume, J., Petitprez, A., Wattel, F., Voisin, C.: Contribution à l'étude des phosphatases acides des macrophages alvéolaires du cobaye. C.R. Soc. Biol. (Paris) 161, 2199–2203 (1967)
Baccino, F.M., Rita, G.A., Maria Franca Zuretti: Studies on the structure-bound sedimentability of some rat liver lysosome hydrolases. Biochem. J. 122, 363–371 (1971)
Bargmann, W., Gaudecker, U.: Über die Ultrastruktur neurosekretorischer Elementargranula. Z. Zellforsch. 96, 495–504 (1969)
Barka, T., Anderson, P.J.: Histochemical methods for acid phosphatases using hexazonium pararosanilin as coupler. J. Histochem. Cytochem. 10, 741–753 (1962)
Burlet, A.: Contribution à l'étude de la synthèse hormonale au niveau du noyau supra-optique du rat dans différentes conditions expérimentales. Détection histochimique et dosage de quelques phosphohydrolases. Thèse Sci. Nancy I, dact. 98f. (1971)
Burlet, A., Marchetti, J., Boulange, M., Legait, E.: Bilan de la vasopressine et modifications enzymologiques du système hypothalamo-neurohypophysaire du rat après surrénalectomie. J. Physiol. (Paris) 65, 207–208 (1972)
Burlet, C.: Variations des activités phosphatasiques acides du système hypothalamo-neurohypophysaire du lérot dans différentes conditions expérimentales. C.R. Soc. Biol. (Paris) 163, 486–488 (1969)
Burlet, C., Burlet, A.: Mise en évidence de plusieurs activités phosphatasiques acides au niveau du système hypothalamo-hypophysaire. C.R. Soc. Biol. (Paris) 161, 2236–2240 (1967)
Cheng, K.W., Friesen, H.G.: Physiological factors regulating secretion of neurophysin. Metabolism 19, 876–890 (1970)
Diamond, M.C.: The effect of hypophysectomy, removal of the posterior pituitary, adrenaleotomy and dehydration on the antidiuretic activity of the rat hypothalamic-neurohypophyseal system. Endocrinology 58, 461–470 (1956)
Duchesne, P.Y.: Quelques aspects histoenzymologiques du système hypothalamo-neurohypophysaire de la souris au cours de la déshydratation. C.R. Soc. Biol. (Paris) 162, 1629–1631 (1968)
Dyball, R.E.J.: Electrical discharge patterns in hypothalamic neurosecretory neurons associated with hormone release. In: Aspects of neuroendocrinology. Ed. by W. Bargmann and B. Scharrer. Berlin-Heidelberg-New York: Springer pp. 153–158. 1970
Eränkö, O.: Histochemical evidence of intense phosphatase activity of the hypothalamic magnocellular nuclei of the rat. Acta physiol. scand. 24, 1–6 (1951)
Flament-Durand, J.: Contribution à l'étude des relations hypothalamo-hypophysaires. Ann. Soc. roy. Sci. méd. nat. Brux. 19, 5–119 (1966)
Forsling, M.L., Martin, M.J., Burton, A.M.: The effect of hydration on vasopressin and neurophysin release in the rat. J. Endocr. 51, 413–414 (1971)
Gerebtzoff, M.A., Maeda, T.: Caractères et localisation histochimique d'un isoenzyme fluoro résistant de la phosphatase acide dans la moelle épinière du rat. C.R. Soc. Biol. (Paris) 162, 2032–2035 (1968)
Gerebtzoff, M.A., Budo, C., Poncelet, G., Schoenen, J.: Localisation histochimique et propriétés d'un isoenzyme fluororésistant de la phosphatase acide dans la moelle épinière du rat et de la souris. C.R. Ass. Anat. 145, 208–214 (1970)
Gharib, C., Berthelon, A., Sassard, J., Dutruge, J.: Le dosage biologique de l'hormone anti diurétique. In: ≪Vasopressine et hypervasopressinismes réactionels≫. pp. 41–60. Sandoz: Rueil-Malmaison 1968
Heller, J., Stulc, J.: The physiology of the antidiuretic hormone. III. The antidiuretic activity of plasma in normal and dehydrated rats. Physiol. bohemoslov. 9, 93–98 (1960)
Jongkind, J.F.: Quantitative histochemistry of hypothalamus. II. Thiamine pyrophosphatase, nucleoside diphosphatase and acide phosphatase in the activated supraoptic nucleus of the rat. J. Histochem. Cytochem. 17, 23–29 (1969)
Jongkind, J.F., Swaab, D.F.: The distribution of thiamine diphosphate-phosphohydrolase in the neurosecretory nuclei of the rat following osmotic stress. Histochemie 11, 319–324 (1967)
Kivalo, E., Rinne, U.K., Mäkelä, S.: Acetylcholinesterase, acid phosphatase and succinic dehydrogenase in the hypothalamic magnocellular nuclei after serotonin administration. Experientia (Basel) 14, 408–409 (1958)
Knyihar, E., Gerebtzoff, M.A.: Localisation ultrastructurale de l'isoenzyme fluororésistant de la phosphatase acide dans la moelle épinière du rat. C.R. Ass. Anat. 149, 786–790 (1970)
Kobayashi, H., Oota, Y., Hirano, T.: Acid phosphatase activity of the hypothalamohypophyseal system of dehydrated rats and pigeons in relation to neurosecretion. Gen. comp. Endocr. 2, 495–498 (1962)
Krsulovic, J., Brückner, G.: Morphological characteristics of pituicytes in different functional stages. Z. Zellforsch. 99, 210–220 (1969)
Legait, E., Legait, H., Roux, M.: Evolution de quelques activités enzymatiques au niveau du système hypothalamo-hypophysaire au cours d'épreuves de déshydratation chez le rat. C.R. Ass. Anat. 132, 649–652 (1965)
Lin, C.W., Fishman, W.H.: Microsomal and lysosomal acid phosphatase isoenzymes of mouse kidney. J. Histochem. Cytochem. 20, 487–498 (1972)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Mirsky, A., Stein, M., Paulisch, G.: The secretion of an antidiuretic substance into the circulation of rats exposed to noxious stimuli. Endocrinology 54, 491–505 (1954)
Nagasawa, J., Douglas, W.W., Schulz, R.A.: Ultrastructural evidence of secretion by exocytosis and of “synaptic vesicle” formation in posterior pituitary glands. Nature (Lond.) 227, 407–409 (1970)
Neil, M.W., Horner, M.W.: The acid p-nitrophenyl phosphate activity of adult guinea-pig liver subcellular fraction. Biochem. J. 84, 32 (1962)
Novikoff, A.B.: Enzyme localization and ultrastructure of neurons. In: “The neuron”. Ed. by H. Hyden. pp. 255–318. Amsterdam: Elsevier 1969
Osinchak, J.: Electron microscopic localization of acid phosphatase and thiamine pyrophosphatase activity in hypothalamic neurosecretory cells of the rat. J. Cell Biol. 21, 35–47 (1964)
Picard, D., Michel-Bechet, M., Athouël, A.M., Rua, S.: Granules neurosécrétoires, lysosomes et complexe GRL dans le noyau supra-optique du rat. Bipolarité des complexes golgiens. Exp. Brain Res. 14, 331–353 (1972)
Pilgrim, C.: Über die Entwicklung des Enzymmusters in den neurosekretorischen hypothalamischen Zentren der Ratte. Histochemie 10, 44–65 (1967)
Pilgrim, C.: Morphologische und funktionelle Untersuchungen zur Neurosekretbildung. Ergebn. Anat. Entwickl.-Gesch. 41, 1–79 (1969)
Pilgrim, C.: Autoradiographische und elektronenmikroskopische Untersuchungen an neurosekretorische Zellen der Ratte unter osmotischer Belastung. Verh. Anat. Ges., Anat. Anz. 126, sup. 10, 69–75 (1970)
Rechardt, L.: Electron microscopic and histochemical observation on the supra-optic nucleus of normal and dehydrated rats. Acta physiol. scand. 77, sup. 329, 79–92 (1969)
Roux, M.: Contribution à l'étude du lobe intermédiaire de l'hypophyse. Etude histophysiologique chez la souris blanche. Thèse Sci., Nancy, dact. 127f. (1967)
Saito, T., Yoshida, S., Nakao, Y.: Release of antidiuretic hormone from neurohypophysis in response to haemorrhage and infusion of hypertonic saline in dogs. Endocrinology 85, 72–78 (1969)
Samorajski, T., Fitz, G.R.: Histochemical analysis of phosphomonoesterase in the hypothalamus and pituitary gland of rat. Lab. Invest. 9, 517–534 (1960)
Santolaya, R.C., Bridges, T.E., Lederis, K.: Elementary granules, small vesicles and exocytosis in rat neurohypophysis after acute haemorrhage. Z. Zellforsch. 125, 277–288 (1972)
Schuel, H., Anderson, Ng.: The distribution of acid phenylphosphatase activities in rat liver brei fractionated in the zonal centrifuge. J. Cell. Biol. 21, 309–323 (1964)
Simon, A.: The pressor and ocytocic content of the hypophysis of rats under various conditions. Amer. J. Physiol. 107, 220–226 (1934)
Sloat, F.B., Allen, J.M.: Soluble and membrane-associated forms of acid phosphatase associated with the lysosomal fraction of rat liver. Ann. N.Y. Acad. Sci. 166, 574–601 (1969)
Tewari, H.B., Thakar, D.S.: On the presence of acid phosphatase at the synapses of the molecular layer of cerebellum of bat and its metabolic significance. Ann. Histochim. 13, 135–140 (1968)
Vangha-Perttula, T.: A new type of acid phosphatase from rat testis. Experientia (Basel) 27, 42–44 (1971)
Vilhard, H.: Vasopressin content and neurosecretory material in the hypothalamo-neurohypophyseal system of rats under different states of water metabolism. Acta Endocr. 63, 585–594 (1970)
Whitaker, S., La Bella, F.S., Sanwal, M.: Electron microscopic histochemistry of lysosomes in neurosecretory nerves endings and pituicytes of rat posterior pituitary. Z. Zellforsch. 111, 493–504 (1970)
Whitaker, S., La Bella, F.S.: Ultrastructural localization of acid phosphatase in posterior pituitary of the dehydrated rat. Z. Zellforsch. 125, 1–15 (1972)
Wislocki, G.B., Dempsey, E.W.: The chemical histology and cytology of the pineal body and neurohypophysis. Endocrinology 42, 56–61 (1948)
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Burlet, A. Estimations histophotométriques et dosages biochimiques des orthophosphorique-monoester-phosphohydrolases acides du système hypothalamo-neurohypophysaire du rat soumis à une épreuve de déshydratation. Exp Brain Res 19, 314–325 (1974). https://doi.org/10.1007/BF00233237
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DOI: https://doi.org/10.1007/BF00233237