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The neuronal organization of horizontal semicircular canalactivated inhibitory vestibulocollic neurons in the cat

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1.The somatic location and axonal projections of inhibitory vestibular nucleus neurons activated by the horizontal semicircular canal nerve (HCN) were studied in anesthetized cats. Cats were anesthetized with ketamine hydrochloride and pentobarbital sodium. 2.Intracellular recordings were obtained from 11 neck extensor motoneurons which were identified by antidromic activation from the dosai rami (DR) in the C1 segment. Stimulation of the ipsilateral (i-) HCN and the ipsilateral abducens (AB) nucleus evoked IPSPs in the motoneurons. These IPSPs were fully or partially occluded when they were evoked simultaneously. 3. Intracellular recordings were obtained from 8 AB motoneurons. Stimulation of the i-HCN and the i-C1DR motoneuron pool evoked IPSPs in the AB motoneurons. These IPSPs were also partially occluded when they were evoked simultaneously, which implied that some HCN-activated neurons inhibit both i-AB motoneurons and ipsilateral neck motoneurons. 4. Unit activity was extracellularly recorded from 30 vestibular neurons that were activated monosynaptically by i-HCN stimulation. Their axonal projections were determined by stimulating the i-AB nucleus and the i-C1DR motoneuron pool. Eight neurons were activated by both stimuli, and were termed vestibulooculo-collic (VOC) neurons. Their axonal branching was examined by means of local stimulation in and around the i-AB nucleus and the i-C1DR motoneuron pool. Eighteen neurons were antidromically activated from the i-C1DR motoneuron pool but not from the i-AB nucleus. These were termed vestibulo-collic (VC) neurons. Four neurons were activated from the i-AB nucleus but not from the ventral funiculus in the C1 segment, and were termed vestibulo-ocular (VO) neurons. The HCN-activated inhibitory neurons were mostly localized in the rostroventral part of the medial vestibular nucleus. 5. Horseradish peroxidase (HRP) was injected iontophoretically into descending axons of 2 HCN-activated inhibitory VOC neurons which were identified by stimulation of the i-HCN and the i-AB nucleus. Axon collaterals were ramified from a stem axon in the ventral funiculus, and entered the gray matter and spread in the laminae VIII and IX. Terminal boutons were distributed over the medial and the ventromedial parts of the vental horn in the C1 segment.

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Authors and Affiliations

  1. Department of Electrical and Electronics Engineering, Faculty of Engineering, Fukui University, 3-9-1Bunkyo, 910, Fukui, Japan

    N. Isu

  2. Department of Oto-laryngology, Saint Marianna University School of Medicine, 2-6-1 Sugao Miyamae, Kawasaki, 213, Kanagawa, Japan

    A. Sakuma

  3. Department of Psychiatry, Tokyo Medical College, 6-7-1 Nishishinjuku Shinjuku-ku, 160, Tokyo, Japan

    K. Hiranuma

  4. Department of Anesthesiology, Tokyo Medical College, 6-7-1 Nishishinjuku Shinjuku-ku, 160, Tokyo, Japan

    H. Uchino

  5. Department of Physiology, Tokyo Medical College, 6-1-1 Shinjuku, Shijuku-ku, 160, Tokyo, Japan

    S -I. Sasaki, M. Imagawa & Y. Uchino

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  1. N. Isu
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  2. A. Sakuma
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  3. K. Hiranuma
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  4. H. Uchino
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  5. S -I. Sasaki
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  6. M. Imagawa
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  7. Y. Uchino
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Isu, N., Sakuma, A., Hiranuma, K. et al. The neuronal organization of horizontal semicircular canalactivated inhibitory vestibulocollic neurons in the cat. Exp Brain Res 86, 9–17 (1991). https://doi.org/10.1007/BF00231035

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  • DOI: https://doi.org/10.1007/BF00231035

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