Summary
We have investigated the effect of alcoholic liver disease (ALD) on the metabolism of 7-ethoxycoumarin 0-deethylase in human liver microsomes. EROD activity was significantly reduced in tissue from ALD patients who smoked, compared to smoking controls. A similar trend was seen in non-smokers. These results have implications for the metabolism of environmental carcinogens.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brodie MJ, Boobis AR, Bulpitt CJ, Davies DS (1981) Influence of liver disease and environmental factors on hepatic monooxygenase activity in vitro. Eur J Clin Pharmacol 20: 39–46
Burke MD, Mayer RT (1983) Differential effects of phenobarbitone and 3-methylcholanthrene induction on the hepatic microsomal metabolism and cytochrome P450 binding of phenoxazone and homologous series of its n alkyl ethers (alkoxy resorufins). Chem Biol Interact 45: 243–258
Johnson PJ, Kraser B, Portman B, Eddleston ALWP, Williams R (1978) Hepatocellular carcinoma in Great Britain: Influence of age, sex, HBsAg status and aetiology of underlying cirrhosis. Gut 19: 1022–1026
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ, (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275
Sims P (1980) The metabolic activation of chemical carcinogents. Br Med Bull 36: 11–18
Thompson S, Petrie JC, Engeset J, Elcombe CR, Mayer RT, von Bahr C, Burke MD (1984) Metabolic evidence for the presence of different forms of cytochrome P-450 in human liver. Biochem Soc Transact 12: 682–683
Williams FM, Mutch E, Woodhouse KW, Lambert D, Rawlins MD (1986) Ethoxyresorufin 0-deethylation by human liver microsomes. Br J Clin Pharmacol 22: 263–268
Woodhouse KW, Williams FM, Mutch E, Wright P, James OFW, Rawlins MD (1983) The effect of alcoholic cirrhosis on the activities of microsomal aldrin epoxidase, 7-ethoxycoumarin 0-deethylase and epoxide hydrolase and on the concentration of reduced glutathione in human liver. Br J Clin Pharmacol 15: 667–672
Woodhouse KW, Williams FM, Mutch E, Wright P, James OFW, Rawlins MD (1984) The effect of alcoholic cirrhosis on the two kinetic components (high and low affinity) of the microsomal 0-deethylation of 7-ethoxycoumarin in human liver. Eur J Clin Pharmacol 26: 61–64
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Woodhouse, K.W., Mutch, E., Williams, F.M. et al. 7-Ethoxyresorufin deethylase (EROD) in human liver —The effect of alcoholic liver disease. Eur J Clin Pharmacol 32, 559–562 (1987). https://doi.org/10.1007/BF02455988
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02455988