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Regional distribution of epidermal growth factor, testosterone and dihydrotestosterone in benign prostatic hyperplasia tissue

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Abstract

In benign prostatic hyperplasia (BPH), basic fibroblast growth factor (bFGF) is found to have a regional distribution, with concentrations in the periurethral zone (where the primitive fibrostromal nodule originates) higher than those of the peripheral subcapsular zone. The aim of the present investigation was to verify whether androgens and epidermal growth factor (EGF) are uniformly distributed from the periurethral to the peripheral zone or whether they show regional differences. Tissue samples, removed by transvesical resection from nine untreated BPH patients, sectioned in periurethral, subcapsular, and intermediate zones, were examined. In the periurethral zone, dihydrotestosterone (DHT), testosterone, and EGF, determined by radioimmunoassay (RIA) techniques after purification on Celite microcolumns and Sep-pak C18 cartridge, showed values significantly higher (mean ± SD: 1121±482 pg, 250±129 pg, and 6.89±3.28 ng/mg DNA, respectively;P<0.01) than those of the subcapsular zone (489±190 pg, 114±70 pg, and 3.40±1.90 ng/mg DNA, respectively). A positive linear correlation between EGF, testosterone, and DHT was also observed. The regional distribution of EGF, testosterone, and DHT was similar to that found for bFGF: the highest levels of these factors in the periurethral region allow us to hypothesize on their possible involvement in the rewakening of mesenchymal tissue, leading to the formation of the primitive fibrostromal nodule and then to BPH development.

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References

  1. Bartsch G, Müller HR, Oberholzer M, Rohr HP (1979) Light microscopic stereological analysis of the normal human prostate and of benign prostatic hyperplasia. J Urol 122:487

    Google Scholar 

  2. Bartsch G, Frick J, Ruegg I (1979) Electron microscopic stereological analysis of the normal human prostate and of benign prostatic hyperplasia. J Urol 122:481

    Google Scholar 

  3. Cunha GR, Chung LWK, Shannon JM, Taguchi O, Fujii H (1983) Hormone-induced morphogenesis and growth: role of mesenchymal-epithelial interactions. Recent Prog Horm Res 39:559

    Google Scholar 

  4. Cunha GR, Donjacour AA, Cooke PS, Mee S, Bigsby RM, Higgins SJ, Sugimura Y (1987) The endocrinology and development biology of the prostate. Endocr Rev 8:338

    Google Scholar 

  5. Deming CL, Neumann C (1939) Early phases of prostatic hyperplasia. Surg Gynecol Obstet 68:155

    Google Scholar 

  6. Fiorelli G, Bellis A de, Longo A, Natali A, Costantini A, Serio M (1989) Epidermal growth factor receptors in human hyperplastic prostate tissue and their modulation by chronic treatment with a gonadotropin-releasing hormone analog. J Clin Endocrinol Metab 68:740

    Google Scholar 

  7. Frydenberg M, Foo TMS, Jones AS, Grace J, Hensley WJ, Rogers J, Pearson BS, Raghavan D (1991) Benign prostatic hyperplasia—video image analysis and its relationship to androgen and epidermal growth factor receptor expression. J Urol 146:872

    Google Scholar 

  8. Griffiths K, Akaza H, Eaton CL, Habib F, Lee C, Robel P, Sciarra F (1991) Hormones, growth factors and benign prostatic hyperplasia (BPH). In: Cockett ATK, Aso Y, Chatelain C, Denis L, Griffiths K, Khoury S, Murphy GP (eds) Proceedings of the international consultation on benign prostatic hyperplasia. S.C.I., Paris, p 25

    Google Scholar 

  9. Griffiths K, Akaza H, Eaton CL, El Etreby M, Habib F, Lee C, Partin AW, Coffey DS, Sciarra F, Steiner G, Tenniswood MP (1993) Regulation of prostatic growth. In: Cockett ATK, Aso Y, Chatelain C, Denis L, Griffiths K, Khoury S, Murphy GP (eds) Proceedings of the 2nd international consultation on benign prostatic hyperplasia. S.C.I., Paris, p 49

    Google Scholar 

  10. Hiramatsu M, Kashimata M, Minami N, Sato A, Murayama M, Minami N (1988) Androgenic regulation of epidermal growth factor in the mouse ventral prostate. Biochem Int 17:311

    Google Scholar 

  11. Lawson R (1991) Prostate growth factors and benign prostatic hyperplasia. Probl Urol 5:449

    Google Scholar 

  12. LeDuc IE (1939) The anatomy of the prostate and the pathology of early benign hypertrophy. J Urol 42:1217

    Google Scholar 

  13. Levine AC, Ren M, Huber GK, Kirschenbaum A (1992) The effect of androgen, estrogen and growth factors on the proliferation of cultured fibroblasts derived from human fetal and adult prostates. Endocrinology 139:2413

    Google Scholar 

  14. Lubrano C, Petrangeli E, Catizone A, Santonati A, Concolino G, Rombolà N, Frati L, Di Silverio F, Sciarra F (1989) Epidermal growth factor binding and steroid receptor content in human benign prostatic hyperplasia. J Steroid Biochem 34:499

    Google Scholar 

  15. Lubrano C, Sciarra F, Spera G, Petrangeli E, Toscano V, Rombolà N, Palleschi F, Palma E, di Silverio F (1992) Immunoreactive EGF in human benign prostatic hyperplasia: relationships with androgen and estrogen receptors. J Steroid Biochem Mol Biol 41:683

    Google Scholar 

  16. Maddy SQ, Chisholm GD, Hawkins RA, Habib FK (1987) Localization of epidermal growth factor receptor assay in the human prostate by biochemical and immunocytochemical methods. J Endocrinol 113:147

    Google Scholar 

  17. McNeal JE (1978) Origin and evolution of benign prostatic enlargement. Invest Urol 15:340

    Google Scholar 

  18. Petrangeli E, Sciarra F, di Silverio F, Toscano V, Lubrano C, Conti C, Concolino G (1988) Effects of two different medical treatments on dihydrotestosterone content and androgen receptors in human benign prostatic hyperplasia. J Steroid Biochem 30:395

    Google Scholar 

  19. Reischauer F (1925) Die Entstehung der sogenannten Prostatahypertrophie. Virchows Arch 256:357

    Google Scholar 

  20. Rouleau M, Leger J, Renniswood M (1990) Ductal heterogeneity of cytokeratins, gene expression and cell death in the rat ventral prostate. Mol Endocrinol 4:2003

    Google Scholar 

  21. Sherwood ER, Fong CJ, Lee C, Kozlowski JM (1992) Basic fibroblast growth factor: a potential mediator of stromal growth in the human prostate. Endocrinology 130:2955

    Google Scholar 

  22. St-Arnaud R, Poyet P, Walker P, Labrie F (1988) Androgens modulate epidermal grwoth factor receptor levels in the rat ventral prostate. Mol Cell Endocrinol 56:21

    Google Scholar 

  23. Toscano V, Petrangeli E, Adamo MV, Foli S, Caiola S, Sciarra F (1981) Simultaneous determination of 5α-reduced metabolites of testosterone in human plasma. J Steroid Biochem 14:574

    Google Scholar 

  24. Traish AM, Wotiz HH (1987) Prostatic epidermal growth factor receptors and their regulation by androgens. Endocrinology 121:1467

    Google Scholar 

  25. Yang Y, Chisholm GD, Habib FK (1993) Epidermal growth factor and transforming growth factor concentrations in BPH and cancer of the prostate: their relationships with tissue androgen levels. Br J Cancer 67:152

    Google Scholar 

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Authors and Affiliations

  1. Istituto di Clinica Medica Generale V, III Cattedra di Endocrinologia, Università degli Studi di Roma “La Sapienza”, Viale del Policlinico, I-00161, Roma, Italy

    F. Sciarra, S. Monti, M. V. Adamo, E. Palma & V. Toscano

  2. Dipartimento di Urologia “U. Bracci”, Cattedra di Urologia, Università degli Studi di Roma “La Sapienza”, Viale del Policlinico, I-00161, Roma, Italy

    G. d'Eramo & F. di Silverio

Authors
  1. F. Sciarra
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  2. S. Monti
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  3. M. V. Adamo
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  4. E. Palma
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  5. V. Toscano
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  6. G. d'Eramo
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  7. F. di Silverio
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Sciarra, F., Monti, S., Adamo, M.V. et al. Regional distribution of epidermal growth factor, testosterone and dihydrotestosterone in benign prostatic hyperplasia tissue. Urol. Res. 23, 387–390 (1995). https://doi.org/10.1007/BF00698740

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  • DOI: https://doi.org/10.1007/BF00698740

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