Abstract
This study used reverse transcription polymerase chain reaction (RT-PCR) to examine heparan sulfate proteoglycan (HS-PG) mRNA expression levels during stone formation in the rat kidney. Total RNA in kidneys was extracted and converted to cDNA. PCR products were resolved by electrophoresis on 1.5% agarose gel and visualized with ethidium bromide. Fragment intensity and area were measured using an image analyzer. Control cyclophilin and HS-PG mRNAs were expressed in all samples examined as 235 by and 506 bp bands, respectively. Cyclophilin expression in the normal group was not significantly different from expression in the group that formed stones. However, the level of HS-PG mRNA expression apparently increased in calcium oxalate (CaOx) microlith. The findings suggest an association between CaOx nephrolithiasis and expression of HS-PG in the rat kidney.
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Barkalow FJB, Schwarzbauer JE (1991) Localization of the major heparin-binding site in fibronectin. J Biol Chem 266:7812
Bernfield M, Kokenyesi R, Kato M, Hinkes MT, Spring J, Gallo RL, Lose EJ (1992) Biology of the syndecans: a family of transmembrane heparan sulfate proteoglycans. Annu Rev Cell Biol 8:365
Boyce WH (1968) Organic matrix of human urinary concretions. Am J Med 45:673
Carey DJ, Evans DM, Stahl RC, Asundi VK, Conner K, Garbes P, Cizmeci SG. (1992) Molecular cloning and characterization of N-syndecan, a novel transmembrane heparan sulfate proteoglycan. J Cell Biol 117:191
Chan VSW, Tan MKL (1995) Determination of heparan sulphate in kidney tissues of patients with calcium nephrolithiasis. Urol Res23:339
Danielson PE, Forss PS, Brow MA, Calavetta L, Douglass J, Milner RJ, Sutcliffe JG (1988) p1B15: A cDNA clone of the rat mRNA encoding cyclophilin. DNA 7:261
Farquhar MG (1981) The glomerular basement membrane: a selective macromolecular filter. In: Hay ED (ed) Cell biology of extracellular matrix. Plenum Press, New York, p 335
Farquhar MG, Lemkin MC, Stow JL (1985) Role of proteoglycans in glomerular function and pathology. In: Robinson RR (ed) Nephrology, vol I. Springer, Berlin Heidelberg New York, p 580
Haendler B, R Hofer WR, Hofer E (1987) Complementary DNA for human T-cell cyclophilin. EMBO J 6:947
Heuvel LPWJ, Born J, Veerkamp JH, Velden TJAM, Schenkels L, Monnens LAH, Schroder CH, Berden JHM (1990) Heparan sulfate proteoglycan from human tubular basement membrane. Comparison with this component from the glomerular basement membrane. Biochim Biophys Acta 1025:67
Hurst RE (1994) Structure, function, and pathology of proteoglycans and glycosaminoglycans in the urinary tract. World J Urol 12:3
Ikeda S, Makino H, Ota Z (1993) Alterations in glomerular extracellular matrix components in glomerulonephritis. Nippon Jinzo Gakkai Shi 35:941
Jalkanen M (1987) Biology of cell surface heparan sulfate proteoglycans. Med Biol 65:41
Kiefer MC, Ishihara M, Swiedler SJ, Crawford K, Stephans JC, Barr PJ (1991) The moleculer biology of heparan sulfate fibroblast growth factor receptors. Ann NY Acad Sci 638:167
Kouzi-Koliakos K, Koliakos GG, Tsilibary EC, Furcht LT, Haronis AS (1989) Mapping of three major heparin-binding sites on laminin and identification of a novel heparin-binding site on the B1 chain. J Biol Chem 264:17971
Nishio S, Abe Y, Wakatsuki A, Iwata H, Ochi K, Takeuchi M, Matsumoto A (1985) Matrix glycosaminoglycan in urinary stones. J Urol 134:503
Nishio S, Satoh A, Yokoyama M, Iwata H, Takeuchi M (1995) Production of heparan sulfate in rat renal tubules in the process of calcium oxalate microlith formation. J Urol 153:352A
Okada Y, Kawamura J, Kuo YJ, Yoshida O (1984) Experimental model for calcium oxalate urolithiasis. In Ryall RL, Brockis JG, Marshall V and Finlayson B (eds) Urinary stone. Churchill Livingstone, New York, p 378
Paulsson M, Fujiwara S, Dziadek M, Timpl R, Pejler G, Backstrom G, Lindahl U, Engel J (1986) Structure and function of basement membrane proteoglycans. In: Evered D, Whelan J (eds) Functions of the proteoglycans. Ciba Foundation symposium 124. Wiley, Chichester, p 189
Rifkin DB, Moscatelli D (1989) Recent developments in the cell biology of basic fibroblast growth factor. J Cell Biol 108:1
Roberts SD, Resnik MI (1986) Glycosaminoglycans content of stone matrix. J Urol 135:1078
Stein PC, Parsons CL (1994) Proteoglycan core protein syndecan in bladder biopsies. World J Urol 12:15
Suzuki K, Mayne K, Doyle IR, Ryall RL (1994) Urinary glycosaminoglycans are selectively included into calcium oxalate crystals precipitated from whole human urine. Scanning Microsc 8:523
Suzuki K, Ryall RL (1996) The effect of heparan sulphate on the crystallization of calcium oxalate in undiluted, ultrafiltered human urine. Br J Urol 78:15
Tamsma JT, Van Den Born J, Bruijn JA, Assmann KJM, Weening JJ, Berden JHM, Wieslander J, Schrama E, Hermans J, Veerkamp JH, Lemkes HHPJ, Van Der Woude FJ (1994) Expression of glomerular extracellular matrix components in human diabetic nephropathy: decrease of heparan sulphate in the glomerular basement membrane. Diabetologia 37:313
Yamaguchi S, Yoshioka T, Utsunomiya M, Koide T, Osafune M, Okuyama A, Sonoda T (1993) Heparan sulfate in the stone matrix and its inhibitory effect on calcium oxalate crystallization. Urol Res 21:187
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Iida, S., Miyajima, J., Suzuki, K. et al. Expression of heparan sulfate proteoglycan mRNA in rat kidneys during calcium oxalate nephrolithiasis. Urol. Res. 25, 361–364 (1997). https://doi.org/10.1007/BF01294666
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DOI: https://doi.org/10.1007/BF01294666