Summary
Previous studies have demonstrated that after intravesical inoculation of Escherichia coli, rats drinking 5% glucose-water remained bacteriuric for up to 21 days while rats drinking tap water became abacteriuric within a few days. To facilitate accurate monitoring of bacteriuria, we created a ventral bladder hernia for percutaneous aspiration of urine. After intravesical inoculation with 108 E. coli, rats with ventral bladder hernias demonstrated clearance of bacteria at a rate comparable to that observed in rats with intrapelvic bladders (p<0.01). Of rats drinking tap water, 6 of 7 (85%) with intrapelvic bladders and 8 of 9 (89%) with ventral hernias had less than 10 colony forming units per ml of urine within 9 days of inoculation. Of rats drinking 5% glucose-water, 4 of 5 (80%) with intrapelvic bladders and 6 of 8 (75%) with ventral bladder hernias had >105 colony forming units per ml of urine 9 days after inoculation. The results suggest that this technique does not alter the antibacterial response of control or polyuric rats to E. coli inoculated intravesically.
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Michaels, E.K., Jarow, J.P. & Schaeffer, A.J. Ventral bladder hernia facilitates study of urinary tract infections in rats. Urol. Res. 9, 249–253 (1981). https://doi.org/10.1007/BF00256896
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DOI: https://doi.org/10.1007/BF00256896