Skip to main content
SpringerLink
Log in

Mouse Ly-31.1 is an alloantigenic determinant of alkaline phosphatase predominantly expressed in the kidney and bone

  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

The mouse lymphocyte surface alloantigen, Ly-31, defined by monoclonal antibody N1.10 (IgG2b, k) and controlled by a gene locus closely linked to theAkp-2 locus on chromosome 4, was biochemically investigated. By employing a quantitative immunoassay system, it was found that the Ly-31.1-specific antibody detected an allotypic determinant of mouse alkaline phosphatase. Ly-31.1, i. e., mouse alkaline phosphatase, was expressed predominantly in kidney and bone and was also detected in placenta, lung, and testis. Concerning tumor cell lines, they varied in the amount of antigen present, with both T and B lymphoid lineages selectively possessing the antigen. In normal lymphoid tissues, lesser amounts of antigen were detected. The binding of mouse alkaline phosphatase to Ly-31.1-specific monoclonal antibodies was specific in nature. The Ly-31.1 antigen was immunoprecipitated from the lysates of surface-radiolabeled YAC-1 moloney leukemia cells, and appeared as a single band of about 78 000 under both reduced and nonreduced conditions on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Furthermore, treatment of tumor cell lines with phosphatidylinositol-specific-phospholipase C resulted in the removal of Ly-31 antigen from the cell surface. These results suggest that a gene cluster containing theLy-31 andAkp-2 loci which control the alkaline phosphatase is formed on mouse chromosome 4. The Ly-31 antigen is the first enzyme demonstrated to be a lymphocyte surface alloantigen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bernstein, I. D.: Joint report of the myeloid section of the second international workshop on human leukocyte differentiation antigens.In E. L. Reinherz, B. F. Haynes, L. M. Nadler, and I. D. Bernstein (eds.):Leukocyte Typing II, Volume 3, pp. 1–25, Springer-Verlag, New York, 1986

    Google Scholar 

  • Boyse, E. A., Miyazawa, M., Aoki, T., and Old, L. J.: Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse.Proc R Soc Lond [Biol] 170: 175–192, 1968

    Google Scholar 

  • Culvenor, J. G., Harris, A. W., Mandel, T. E., Whitelaw A., and Ferber, E.: Alkaline phosphatase in hematopoietic tumor cell lines of the mouse: high activity in cells of the B lymphoid lineage.J Immunol 126: 1974–1977, 1981

    Google Scholar 

  • Fishman, W. H.: Oncotrophoblast gene expression: placental alkaline phosphatase.Adv Cancer Res 48: 1–35, 1987

    Google Scholar 

  • Garattini, E., Hua, J.-C., Pan, Y.-C. E., and Udenfriend, S.: Human liver alkaline phosphatase, purification and partial sequencing: homology with the placental isozyme.Arch Biochem Biophys 245: 331–337, 1986

    Google Scholar 

  • García-Rozas, C., Plaza, A., Díaz-espada, F., Kreisler, M., and Martínez-Alonso, C.: Alkaline phosphatase activity as a membrane marker for activated B cells.J Immunol 128: 52–55, 1982

    Google Scholar 

  • Goffinet, G., Houben-Defresne, M. P., and Boniver, J.: Correlation of alkaline phosphatase activity to normal T-cell differentiation and to radiation leukemia virus-induced preleukemic cells in the C57BL mouse thymus.Cancer Res 43: 5416–5426, 1983

    Google Scholar 

  • Haynes, B. F.: Summary of T cell studies performed during the second international workshop and conference on human leukocyte differentiation antigens.In E. L. Reinherz, B. F. Haynes, L. M. Nadler, and I. D. Bernstein (eds.):Leukocyte Typing II, Volume 1, pp. 3–30, Springer-Verlag, New York, 1986

    Google Scholar 

  • Haziot, A., Chen, S., Ferrero, E., Low, M. G., Siber, R., and Goyert, S. M.: The monocyte differentiation antigen, CD14, is anchored to the cell membrane by a phosphatidylinositol linkage.J Immunol 141: 547–552, 1988

    Google Scholar 

  • Henthorn, P. S., Raducha, M., Edwards, Y. H., Weiss, M. J., Slaughter, C., Lafferty, M. A., and Harris, H.: Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: closely homology to placental alkaline phosphatase.Proc Natl Acad Sci USA 84: 1234–1238, 1987

    Google Scholar 

  • Holmes, K. L., Palfree, R. G. E., Hämmerling, U., and Morse, H. C. III.: Alleles of the Ly-17 alloantigen define polymorphisms of the murine IgG Fc receptor.Proc Natl Acad Sci USA 82: 7706–7710, 1985

    Google Scholar 

  • Hood, L., Campbell, J. H., and Elgin, S. C. R.: The organization, expression and evolution of antibody genes and other multigene families.Annu Rev Genet 8: 305–353, 1975

    Google Scholar 

  • Jemmerson, R. and Fishman, W. H.: Convenient selection of monoclonal antibodies to isoenzymes of placental alkaline phosphatase using the catalytic activity of the antigen in enzymeantigen immunoassay.Anal Biochem 124: 286–292, 1982

    Google Scholar 

  • Kam, W., Clauser, E., Kim, Y. S., Kam, Y. W., and Rutter, W. J.: Cloning, sequencing, and chromosomal localization of human term placental alkaline phosphatase cDNA.Proc Natl Acad Sci USA 82: 8715–8719, 1985

    Google Scholar 

  • Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227: 680–685, 1970

    Google Scholar 

  • Low, M. G. and Finean, J. B.: Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C.Biochem J 167: 281–284, 1977

    Google Scholar 

  • Low, M. G. and Kincade, P. W.: Phosphatidylinositol is the membraneanchoring domain of the Thy-1 glycoprotein.Nature 318: 62–64, 1985

    Google Scholar 

  • Nadler, L. M.: B cell/leukemia panel workshop: summary and comments.In E. L. Reinherz, B. F. Haynes, L. M. Nadler, and I. D. Bernstein (eds.):Leukocyte Typing II, Volume 2, pp. 3–43, Springer-Verlag, New York, 1986

    Google Scholar 

  • Nakamura, S., Ikegami, S., Dairiki, K., Kochiya, M., Fujimori, T., Tamaoki, N., and Tada, N.: Biochemical similarity of Ly-19, Ly-32, and Lyb-2 alloantigens encoded in the gene cluster on mouse chromosome 4.Immunogenetics 28: 314–319, 1988

    Google Scholar 

  • Neumann, H., Wilson, K. J., Hauck-Granoth, R., and Haran-Ghera, N.: A comparative biochemical study of alkaline phosphatase in normal and leukemic mice.Cancer Res 31: 1695–1701, 1971

    Google Scholar 

  • Neumann, H., Klein, E., Hauck-Granoth, R., Yachnin, S., and BenBassat, H.: Comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells.Proc Natl Acad Sci USA 73: 1432–1436, 1976

    Google Scholar 

  • Reif, A. E. and Allen, J. M. V.: Specificity of isoantisera against leukemic and thymic lymphocytes.Nature 200: 1332–1333, 1963

    Google Scholar 

  • Ronai, A., Wassmer, B., de Looze, S., and van Deimling, O.: Immunochemical interrelationships between carboxylesterase isozymes (EC 3.1.1.1) of the house mouse,Mus musculus.Camp Biochem Physiol 82B: 347–355, 1985

    Google Scholar 

  • Slein, M. W. and Logan, G. F.: Characterization of the phospholipases of Bacillus cereus and their effects on erythrocytes, bone, and kidney cells.J Bacteriol 90: 69–81, 1965

    Google Scholar 

  • Smith, I., Lightstone, P. J., and Perry, J. D.: Separation of human tissue alkaline phosphatases by electrophoresis on acrylamide disc gels.Clin Chim Acta 18: 499–505, 1968

    Google Scholar 

  • Swallow, D. M., Povey, S., Parkar, M., Andrews, P. W., Harris, H., Pym, B., and Goodfellow, P.: Mapping of the gene coding for the human liver/bone/kidney isozyme of alkaline phosphatase to chromosome 1.Ann Hum Genet 50: 229–235, 1986

    Google Scholar 

  • Tada, N., Kimura, S., and Hämmerling, U.: Gene-cluster formation of mouse alloantigen loci. A survey with monoclonal antibodies.In G. J. Hämmerling, and J. Kearney (eds.):Monoclonal Antibodies and T Cell Hybridomas, pp. 38–44, Elsevier, North Holland, Amsterdam, 1981

    Google Scholar 

  • Tada, N., Kimura, S., Liu-Lam, Y., and Hämmerling, U.: A new lymphocyte surface antigen (Ly-m31) controlled by a gene closely link ed to theAkp-2 locus on mouse chromosome 4.Immunogenetics 20: 589–592, 1984

    Google Scholar 

  • Tada, N., Tamaoki, N., Ikegami, S., Nakamura, S., Dairiki, K., and Fujimori, T.: TheLyb-2-Ly-19 region of mouse chromosome 4 controls a new cell-surface alloantigen: Ly-32.Immunogenetics 25: 269–270, 1987

    Google Scholar 

  • Terao, M. and Mintz, B.: Cloning and characterization of a cDNA coding for mouse placental alkaline phosphatase.Proc Natl Acad Sci USA 84: 7051–7055, 1987

    Google Scholar 

  • Terao, M., Pravtcheva, D., Ruddle, F. H., and Mintz, B.: Mapping of gene encoding mouse placental alkaline phosphatase to chromosome 4.Somatic Cell Mol Genet 14: 211–215, 1988

    Google Scholar 

  • Weiss, M. J., Henthorn, P. S., Lafferty, M. A., Slaughter, C., Raducha, M., and Harris, H.: Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase.Proc Natl Acad Sci USA 83: 7182–7186, 1986

    Google Scholar 

  • Wilcox, F. H.: Genetics of alkaline phosphatase of the small intestine of the house mouse (Mus musculus).Biochem Genet 21: 641–652, 1983

    Google Scholar 

  • Wilcox, F. H. and Taylor, B. A.: Genetics of theAkp-2 locus for alkaline phosphatase of liver, kidney, bone, and placenta in the mouse.J Hered 72: 387–390, 1981

    Google Scholar 

  • Wilcox, F. H., Hirschhorn, L., Taylor, B. A., Womack, J. E., and Roderick, T. H.: Genetic variation in alkaline phosphatase of the house mouse (Mus musculus) with emphasis on a manganese-requiring isozyme.Biochem Genet 17: 1093–1107, 1979

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Immunogenetics, Meiji Institute of Health Science, 540 Naruda, 250, Odawara, Japan

    Kazuo Dairiki, Shuji Nakamura, Shuji Ikegami, Madoka Nakamura & Takao Fujimori

  2. Department of Pathology, Tokai University School of Medicine, 259-11, Isehara, Kanagawa, Japan

    Norikazu Tamaoki & Nobuhiko Tada

Authors
  1. Kazuo Dairiki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shuji Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shuji Ikegami
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Madoka Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takao Fujimori
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Norikazu Tamaoki
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Nobuhiko Tada
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dairiki, K., Nakamura, S., Ikegami, S. et al. Mouse Ly-31.1 is an alloantigenic determinant of alkaline phosphatase predominantly expressed in the kidney and bone. Immunogenetics 29, 235–240 (1989). https://doi.org/10.1007/BF00717907

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00717907

Keywords

Search

Navigation