Summary
The growth characteristics of the yeastCandida utilis in the individual stages of a multistage tower fermentor obtained with single- and multistream ethanol feeding were compared. In addition, various types of pure oxygen supply were tested for each type of ethanol feed. The results, obtained from steady-state continuous cultures, provided evidence that the two types of ethanol and oxygen supply significantly affect the cell growth rate, ethanol dissimilation rate, acetate excretion in the medium, biomass yield and productivity.
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References
Abbott BJ (1973) Ethanol inhibition of a bacterium Acinetobacter calcoaceticus in Chemostat culture. J. Gen. Microbiol. 75:383–389
Aiba S, Shoda M, Nagatani M (1968) Kinetics of product inhibition in alcohol fermentation. Biotechnol Bioeng 10:845–864
Brown SW, Oliver SG, Harrison DEF, Righelato RC (1981) Ethanol inhibition of yeast growth and fermentation: Differences in the magnitude and complexity of the effect. Eur J Appl Microbiol Biotechnol 11:151–155
Day A, Anderson E, Martin PA (1975) Ethanol tolerance of brewing yeast. Eur. Brew. Conv. Proc. Congr. 377–391
Ghose TK, Tyagi RD (1979) Rapid ethanol fermentation of cellulose hydrolysate. II. Product and substrate inhibition and optimalization of fermentor design. Biotechnol Bioeng 21:1401–1420
Gleiser IE, Bauer S (1981) Growth ofE. coli W to high cell concentration by oxygen level linked control of carbon source concentration. Biotechnol Bioeng 23:1015–1021
Holzberg I, Finn RK, Steinkraus KH (1967) A kinetic study of the alcoholic fermentation of grape juice. Biotechnol Bioeng 9:413–427
Huňková Z, Fencl Z (1977) Toxic effects of fatty acids on yeast cells: Dependence of inhibitory effects on fatty acid concentration. Biotechnol Bioeng 19:1623–1641
Ingram LO, Vreeland NS (1980) Differential effect of ethanol and hexanol on theEscherichia coli cell envelope
Ingram LO (1981) Mechanism of lysis ofEscherichia coli by ethanol and other chaotropie agents. J Bacteriol 146:331–336
Johnson MJ (1967) Aerobic microbial growth at low oxygen concentrations. J Bacteriol 94:101–108
Mor JR, Fiechter A (1968a) Continuous cultivation of Saccharomyces cerevisiae. I. Growth on ethanol under steady-state conditions. Biotechnol Bioeng 10:159–176
Mor JR, Fiechter A (1968b) Continuous cultivation of Saccharomyces cerevisiae. II. Growth of ethanol under transient-state conditions. Biotechnol Bioeng 10:787–800
Novák M, Strehaiano P, Moreno M, Goma G (1981) Alcoholic fermentation: On the inhibitory effect of ethanol. Biotechnol Bioeng 23:201–211
Omata S, Murao S, Terashima H (1970) J Ferment Ass (Japan) 26:317–327
Páca J (1981) Effect of Multistream ethanol feeding on growth and physiological characteristics of Candida utilis in a multistage tower fermentor. Enzyme Microbiol Technol 3:123–128
Páca J (1980a) Elimination of ethanol inhibition of yeast growth by a multistream ethanol feed in a multistage tower fermentor. J Chem Technol Biotechnol 30:764–771
Páca J (1980b) Induction effect of pO2 during continuous yeast production from ethanol in a multistage tower fermentor. Eur J Appl Microbiol Biotechnol 9:93–100
Páca J, Grégr V (1979a) Effect of PO2 growth and physiological characteristics of Candida utilis in a multistage tower fermentor. Biotechnol Bioeng 21:1827–1843
Páca J, Grégr V (1979b) Growth characteristics of Candida utilis in a multistage culture system. Enzyme Microbiol Technol 1:100–106
Páca J, Grégr V (1979c) Effect of interstage mixing in multistage culture systems on continuous biomass production. Biotechnol Bioeng 21:1809–1825
Páca J, Grégr V (1976) Design and performance characteristics of a continuous Multistage tower fermentor. Biotechnol Bioeng 18:1075–1090
Prior B, Kilian S, Lategan P (1980) Growth of Candida utilis on ethanol and isopropanol. Arch Microbiol 125:133–136
Prokop A, Votruba J, Sobotka M, Panoš J (1978) Yeast SCP from ethanol: Measurements. modeling and parameter estimation in a batch system. Biotechnol Bioeng 20:1523–1540
Rogers PJ, Stewart PR (1973) Mitochondrial and peroxisomal contributions to the energy metabolism of Saccharomyces cerevisiae in continuous culture. J Gen Microbiol 79:205–217
Thomas DS, Rose AH (1979) Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasma. membrane liquid composition. Arch Microbiol 122:49–55
Unger P, Vozňáková Z, Páca J (1977) Analysis of cell metabolic products and fermentation gases by gas chromatography. J Appl Chem Biotechnol 27:150–154
Watteeuw CM, Armiger WB, Ristroph DL, Humphrey AE (1979) Production of single cell protein from ethanol by fed-batch process. Biotechnol. Bioeng. 21:1221–1237
Zines DO, Rogers PL (1970) The effect of ethanol on continuous culture stability. Biotechnol. Bioeng. 12:561–575
Zines DO, Rogers PL (1971) A chemostat study of ethanol inhibition. Biotechnol Bioeng 13:293–308
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Páca, J. Comparison of ethanol feed-type on yeast growth at various po2 levels. European J. Appl. Microbiol. Biotechnol. 15, 9–13 (1982). https://doi.org/10.1007/BF01875393
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DOI: https://doi.org/10.1007/BF01875393