Summary
Immobilization of Saccharomyces cerevisiae as a monocellular layer adhering to a glass support has been achieved by adsorbing Al or Fe(III) ions on the cells or by coating the support with Al(OH)3 or Fe2O3 particles. The initial rate of glucose to ethanol conversion was measured in pure glucose solutions for free cells in suspension, either untreated or submitted to the chemical treatments used for immobilization, and for immobilized cells, using a small reactor (50–70 ml) in closed circuit. The conversion rate of immobilized cells is in the range of 3.5 to 5×10-14 mol/cell·h while that of untreated suspended cells is in the range of 5 to 7×10-14 mol/cell·h.
Similar content being viewed by others
References
Anderson MA, Palm-Gennen MH, Renard PN, Defossé C, Rouxhet PG (1984) Chemical and XPS study of the adsorption of iron(III) onto porous silica. J Colloid Interface Sci 102:328–336
Chibata I, Wingard LB (eds) (1983) Immobilized Microbial Cells, Vol. 4 in Applied Biochemistry and Bioengineering, Wingard LB, Goldstein L, Katchalski-Katzir E (eds), Academic Press, New-York-London-Toronto-Sydney-San Francisco
De Bremaeker M, Gennen M, Kayem, GJ, Van Haecht JL, Rouxhet PG (1980) Procédé d'immobilisation de cellules microbiennes globulaires par adhésion à un support solide, Belgian Patents No 884.877 and 884.878
Kayem GJ, Rouxhet PG (1983) Adsorption of colloidal hydrous alumina on yeast cells. J Chem Soc, Faraday Trans I, 79:561–569
Kayem GJ, Palm-Gennen MH, Rouxhet PG (1985) Adhesion of yeast on glass coated with a colloidal oxide. (in press)
Klibanov AM (1983) Immobilized enzymes and cells as practical catalysts. Science 219:722–727
Linko YY, Linko P (1981) Continuous ethanol fermentation with immobilized yeast. 2nd European Congress of Biotechnology 1981, Eastbourne, Abstracts of Communications, p 56
Navarro JM, Durand G (1977) Modification of yeast metabolism by immobilization onto porous glass. European J Appl Microbiol 4:243–254
Rouxhet PG, Van Haecht JL, Didelez J, Gerard P, Briquet M (1981) Immobilization of yeast cells by entrapment and adhesion using siliceous materials. Enzym Microb Technol 3:49–54
Rouxhet PG, Mozes N, Van Haecht JL, Reuliaux L, Palm-Gennen MH (1984) Immobilization of microbial cells by adhesion to a support. 3rd European Congress of Biotechnology, Verlag Chemie, Vol. I, pp 319–325
Van Haecht JL, De Bremaeker M, Rouxhet PG (1984) Immobilization of yeast by adhesion to a support without use of a chemical agent. Enzym Microb Technol 6:221–227
Van Haecht JL, Bolipombo M'Bombo, Rouxhet PG (1985) Adsorption of Al ions by yeast and immobilization of the cells by adhesion to glass. Biotechnol Bioeng (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mozes, N., Rouxhet, P.G. Metabolic activity of yeast immobilized as supported monolayer. Appl Microbiol Biotechnol 22, 92–97 (1985). https://doi.org/10.1007/BF00250026
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00250026