Summary
Ligninase activity of Phanerochaete chrysosporium INA-12 was increased when vegetable oils emulsified with sorbitan polyoxyethylene monooleate (Tween 80) were added to growth medium. Maximal enzyme yield was 22.0 nkat·ml-1 in olive oil cultures after 4 days incubation. P. chrysosporium INA-12 was also able to utilize tall oil fatty acids for ligninase synthesis. An extracellular lipase activity was detected during the primary phase of growth in culture containing vegetable oils. On the other hand, ligninase production was 1.5-fold enhanced when olive oil cultures were supplemented with soybean asolectin as a phospholipid source. In cultures supplied with olive oil plus asolectin, P. chrysosporium INA-12 mycelium exhibited a preferential enrichment of oleic acid (C18:1), phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) as compared to lipid-free medium. PC and LPC enrichment was associated with an increased ratio of saturated versus unsaturated fatty acids of phospholipids.
Similar content being viewed by others
References
Adler L, Liljenberg C (1981) Sterol content, fatty acid composition of phospholipids and permeability of labeled ethylene glycols in relation to salt-tolerance of yeasts. Physiol Plant 53:368–374
Asther M, Corrieu G, Drapron R, Odier E (1987) Effect of Tween 80 and oleic acid on ligninase production by Phanerochaete chrysosporium INA-12. Enz Microbial Technol 9:245–249
Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234:466–468
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Buswell JA, Mollet B, Odier E (1984) Ligninolytic enzyme production by Phanerochaete chrysosporium under conditions of nitrogen sufficiency. FEMS Microbiol Lett 25:295–299
Chopra A, Khuller GK (1983) Lipids of pathogenic fungi. Prog Lipid Res 22:189–220
Conner AH, Rowe JW (1975) Neutrals in southern prine tall oil. J Am Oil Chem Soc 52:334–338
Desnuelle P, Constantin MJ, Baldy J (1955) Technique potentiométrique pour la mesure de l'activité de la lipase pancréatique. Bull Soc Chim Biol 37:285–287
Eggstein M, Kuhlmann E (1974) Triglycerides and glycerol determination after alkaline hydrolysis. In: Bergmeyer HU (ed), Methods of enzymatic analysis. Academic Press, London, pp 1825–1831
Faison BD, Kirk TK (1985) Factors involved in the regulation of a ligninase activity in Phanerochaete chrysosporium. Appl Environ Microbiol 49:299–304
Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509
Gold MH, Kuwahara M, Chiu AA, Glenn JK (1984) Purification and characterization of an extracellular H2O2-requiring diarylpropane oxygenase from the white-rot basidiomycete. Phanerochaete chrysosporium. Arch Biochem Biophys 234:353–362
Jäger A, Croan S, Kirk TK (1985) Production of ligninases and degradation of lignin in agitated submerged cultures of Phanerochaete chrysosporium. Appl Environ Microbiol 50:1274–1278
Kates M, Hagen PO (1964) Influence of temperature on fatty acid composition of psychrophilic and mesophilic Serratia spp. Can J Biochem Physiol 42:481–488
Kirk TK, Croan S, Tien M, Murtagh KE, Farell RL (1986) Production of multiple ligninases by Phanerochaete chrysosporium: effect of selected growth conditions and use of a mutant strain. Enzyme Microbial Technol 8:27–32
Ksandopulo GB (1974) Influence of certain fats and surface-active substances on the lipase activity of fungi of the genus Geotrichum. Mikrobiologiya 43:1001–1004
Leisola MSA, Thanei-Wyss U, Fiechter A (1985) Strategies for production of high ligninase activities by Phanerochaete chrysosporium. J Biotechnol 3:97–107
Marion D, Gandemer G, Douillard R (1984) Separation of plant phosphoglycerides and galoctosylglycerides by high performance liquid chromatography. In: Siegenthaler PA, Eichenberger (eds) Structure, function and metabolism of plant lipids. Elsevier Science Publishers B. V., pp 139–143
Morrison WR, Smith LM (1964) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J Lipid Res 5:600–608
Orndorff SA (1986) Enzymatic and fermentative production of speciality chemicals from tall oil. In: Biotechnology in the pulpe and paper industry, Swedish Forest Products Research Laboratory, Stockholm Sweden, pp 114–116
Rao TVG, Trivedi A, Prasad R (1985) Phospholipid enrichment of Saccharomyces cerevisiae and its effect on polyene sensitivity. Can J Microbiol 31:322–326
Thomas DS, Hossak JA, Rose AH (1978) Plasma-membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae. Arch Microbiol 117:239–245
Tien M, Kirk TK (1984) Lignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization and catalytic properties of a unique H2O2-requiring oxygenase Proc Natl Acad Sci USA 81:2280–2284
Wijeyaratne SC, Ohta K, Chavanich S, Mahamontri V, Nilubol N, Hayashida S (1986) Lipid composition of a Thermotolerant yeast Hansenula polymorpha. Agric Biol Chem 50:827–832
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Asther, M., Lesage, L., Drapron, R. et al. Phospholipid and fatty acid enrichment of Phanerochaete chrysosporium INA-12 in relation to ligninase production. Appl Microbiol Biotechnol 27, 393–398 (1988). https://doi.org/10.1007/BF00251775
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00251775