Summary
The feasibility of pressurized culture was explored for the growth of Aureobasidium pullulans and the synthesis of pullulan. For all volumetric flow rates of air, the production of biomass increased with pressure up to a critical value ranging from 0.50 to 0.75 MPa, at which point a drastic decrease in biomass production and a change in cellular morphology was observed. For pullulan synthesis, the same dramatic decrease was observed at approximately the same critical pressure. In the pressure range 0.1–0.65 MPa, the synthesis of pullulan was subject to what is believed to be the competing effects of oxygen availability and pressure inhibition.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arcay-Ledzema GJ, Slaughter JC (1984) The response of Saccharomyces cerevisiae to fermentation under carbon dioxide pressure. J Inst Brew 90:81–84
Bailey JE, Ollis DF (1986) Biochemical engineering fundamentals, 2nd edn. McGraw-Hill, Toronto
Finn RK (1966) Inhibitory cell products: their formation and some new methods of removal. J Ferment Technol 44:305–310
Ho CS, Smith MD (1986) Effect of dissolved carbon dioxide on penicillin fermentations: mycelial growth and penicillin production. Biotechnol Bioeng 28:668–677
Ichikawa Y, Sato S, Takahashi J (1981) Effect of pressure on biomass production from n-butane. J Ferment Technol 59:269–273
Jones RP, Greenfield PF (1982) Effect of carbon dioxide on yeast growth and fermentation. Eng Microbiol Technol 4:210–223
King AD, Nagel CW (1975) Influence of carbon dioxide upon the metabolism of Pseudomonas aeruginosa. J Food Sci 40:362–366
LeDuy A, Zajic JE, Luong JHT, Choplin L (1988) Pullulan. In: Mark HF, Bikales NM, Overberger CG, Menges G, Kroschwitz JI (eds) Encyclopedia of polymer science and engineering, vol 13, 2nd edn. Wiley, Toronto, pp 650–660
Mulchandani A, Luong JHT, LeDuy A (1989) Biosynthesis of pullulan using immobilized Aureobasidium pullulans cells. Biotechnol Bioeng 33:306–312
Peterson RE (1970) Measurement of the solubility of gases in oils. Fed Proc 29:1714–1716
Rho D, Mulchandani A, Luong JHT, LeDuy A (1988) Oxygen requirement in pullulan fermentation. Appl Microbiol Biotechnol 28:361–366
Sato S, Mukataka S, Kataoka H, Takahashi J (1984) Effects of pressure and dissolved oxygen concentration on growth of Pseudomonas aeruginosa. J Ferment Technol 62:71–75
Seeman P (1972) The membrane actions of anaesthetics and tranquilizers. Pharmacol Rev 24:583–655
Smith MD, Ho CS (1985) The effect of carbon dioxide on penicillin production: mycelial morphology. J Biotechnol 2:347–363
Takamatsu T, Shioya S, Nakatani H (1981) Analysis of a deep U-tube aeration system from the viewpoint of oxygen transfer. J Ferment Technol 59:287–294
Yuen S (1974) Pullulan and its applications. Process Biochem 9:7–9, 22
Ziegler H, Dunn IJ, Bourne JR (1980) Oxygen transfer and mycelial growth in a tubular loop fermentor. Biotechnol Bioeng 22:1613–1635
Author information
Authors and Affiliations
Additional information
Offprint requests to: J. Thibault
Rights and permissions
About this article
Cite this article
Dufresne, R., Thibault, J., Leduy, A. et al. The effects of pressure on the growth of Aureobasidium pullulans and the synthesis of pullulan. Appl Microbiol Biotechnol 32, 526–532 (1990). https://doi.org/10.1007/BF00173722
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00173722