Summary
Erwinia chrysanthemi cells were used to study the possibility of producing bacterial enzymes in a bioreactor coupled with a membrane filtration unit. Continuous fermentations with total cell recycle failed to give good production of pectate lyase (PL). Enzymatic, mechanical and physico-chemical damages were involved in this phenomenon. With a sequential recycle mode, we obtained productivity of 1.5 units·h−1·1−1 with a high PL concentration. Protease accumulation occurred when the bioreactor was coupled to a filtration unit. Moreover we have observed no loss of activity due to high shear stress caused by pumping.
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Andro T, Chambost JP, Kotoujansky A, Cattaneo J, Bertheau Y, Barras F, Gijsegem F van, Coleno A (1984) Mutants of Erwinia chrysanthemi defective in secretion of pectinase or cellulase. J Bacteriol 160:1199–1203
Aunstrup K, Andresen O, Falch EA, Nielsen TK (1979) Production of microbial enzymes. In: Peppler HJ, Perlman D (eds) Microbial technology, vol 1, 2nd edn. Academic Press, New York, pp 281–309
Bertheau Y, Madgidi-Hervan E, Kotoujansky A, Nguyen-The C, Andro T, Coleno A (1984) Detection of polymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves. Anal Biochem 139:383–389
Beyeler W, Rogers PL, Fiechter A (1984) A simple technique for the direct determination of maintenance energy coefficient: an example with Zymomonas mobilis. Appl Microbiol Biotechnol 19:277–280
Boyaval P, Goulet J (1988) Optimal conditions for production of lactic acid from cheese whey by Ca-alginate entrapped Lactobacillus helveticus. Enzyme Microb Technol 10:725–728
Boyaval P, Corre C, Terre S (1987) Continuous lactic acid fermentation with concentrated product recovery by ultrafiltration and electrodialysis. Biotechnol Lett 9:207–212
Charm S, Wong B (1970) Shear degradation of fibrinogen in the circulation. Science 170:466–468
Charm S, Wong B (1981) Shear effects on enzymes. Enzyme Microb Technol 3:111–118
Cheryan M, Mehaia MA (1983) A high performance membrane bioreactor for continuous fermentation of lactose to ethanol. Biotechnol Lett 5:519–524
Goma G, Uribelarrea JL, Soucaille P, Minier M, Ferras E (1984) High cell concentration cultures: physiological factors affecting their achievement and technological solutions. In: Third European Congress on Biotechnology München, 10–14 Sept 1984, vol 2, Verlag Chemie, Weinheim pp 451a–451c
Hjörleifsdottir S, Seevaratnam S, Holst O, Mattiasson B (1990) Effects of complete cell recycling on product formation by Lactobacillus casei ssp. rhamnosus in continuous cultures. Curr Microbiol 20:287–292
Holst O, Hansson L, Berg AC, Mattiasson B (1985) Continuous culture with complete cell recycle to obtain high cell densities in product inhibited cultures: cultivation of Streptococcus lactis for production of superoxide dismutase. Appl Microbiol Biotechnol 23:10–14
Jauneau A, Morvan C, Fenyo JC, Demarty M (1988) Pectate lyase production by Bacillus subtilis in a membrane bioreactor. Appl Microbiol Biotechnol 29:234–238
Kotoujansky A, Diolez A, Boccara M, Bertheau Y, Andro T, Coleno A (1985) Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes. EMBO J 4:781–785
Lockwood LB (1979) Production of organic acids by fermentation. In: Peppler HJ, Perlman D (eds) Microbial technology, vol 1, 2nd edn. Academic Press, New York, pp 355–387
Prigent C, Corre C, Boyaval P (1988) Production of concentrated Streptococcus salivarius subsp. thermophilus by coupling continuous fermentation and ultrafiltration. J Dairy Res 55:569–577
Tutunjian R (1985) Ultrafiltration processes in biotechnology. In: Moo Young M (ed) Comprehensive biotechnology, vol 2, Pergamon Press, Oxford, pp 411–437
Virkar P, Narendranathan T, Hoare M, Dunnil P (1981) Studies of the effects of shear on globular proteins: extension to high shear fields and to pumps. Biotechnol Bioeng 23:425–429
Wandersman C, Delepelaire P, Letoffe S, Schwartz M (1987) Characterization of Erwinia chrysanthemi extracellular proteases: cloning and expression of the protease genes in Escherichia coli. J Bacteriol 169:5046–5053
Wang DIC, Sinskey AJ, Butterworth TA (1970) Enzyme processing using ultrafiltration membranes. In: Flinn E (ed) Membrane science and technology. Plenum Press, New York, pp 98–119
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Denis, S., Boyaval, P. Microbial enzyme production in a membrane bioreactor. Appl Microbiol Biotechnol 34, 608–612 (1991). https://doi.org/10.1007/BF00167908
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DOI: https://doi.org/10.1007/BF00167908