Abstract
The use of a phosphate organic form taken up by Kluyveromyces lactis removes repression of the PHO5 promoter and releases heterologous interleukin 1\ synthesis while providing sufficient phosphate for growth. The oxidative metabolism of high-cell-density fed-batch and chemostat cultures was thus maintained under derepressed protein synthesis conditions. Interleukin 1\ production was then growth-associated, an unusual mode of protein synthesis regulation under the control of the PHO5 promoter.
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Blondeau, K., Boutur, O., Boze, H. et al. Development of high-cell-density fermentation for heterologous interleukin 1\ production in Kluyveromyces lactis controlled by the PH05 promoter. Appl Microbiol Biotechnol 41, 324–329 (1994). https://doi.org/10.1007/BF00221227
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DOI: https://doi.org/10.1007/BF00221227