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Characterization of a bacteriocin produced by Streptococcus thermophilus ST134

  • Applied Genetics and Regulation
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Abstract

A pH-dependent adsorption/desorption technique was used to screen Streptococcus thermophilus strains for the production of bacteriocins. Agar-diffusion tests with S. thermophilus strains as targets identified 13 out of 41 strains as producers of antibacterial activity. Thermophilin A, the bacteriocin-like substance present in the culture supernatant of S.thermophilus ST134 was purified to homogeneity by ammonium sulfate precipitation and ion-exchange chromatography, followed by ultrafiltration. Thermophilin A is a relatively heat-stable and apparently glycosylated bacteriocin with a bactericidal mode of action against sensitive cells.

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Authors and Affiliations

  1. U.S. Department of Agriculture, ARS, Eastern Regional Research Center, 600 E. Mermaid Lane, 19118, Philadelphia, PA, USA

    D. J. Ward & G. A. Somkuti

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  1. D. J. Ward
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  2. G. A. Somkuti
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Ward, D.J., Somkuti, G.A. Characterization of a bacteriocin produced by Streptococcus thermophilus ST134. Appl Microbiol Biotechnol 43, 330–335 (1995). https://doi.org/10.1007/BF00172834

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  • DOI: https://doi.org/10.1007/BF00172834

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