Skip to main content
SpringerLink
Log in

Immunotherapy of a guinea pig hepatoma with ultrasonically prepared mycobacterial vaccines

  • Short Communications
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Summary

Oil-in-water emulsions containing either mycobacterial cell walls (CW) or killed whole cells attached to the oil droplets were prepared by ultrasonication and by a modified grinding procedure. The immunotherapeutic potency of the ultrasonically prepared vaccines was at least as great as that of similar vaccines prepared by emulsification in a tissue grinder. Among the advantages of ultrasonication over grinding for the preparation of mycobacterial cell and cell wall vaccines are simplicity and the ease with which sterility may be maintained. Both the ultrasonic and modified grinding methods are less time-consuming than published procedures for preparing mycobacterial vaccines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Baldwin, R. W., Cook, A. J., Hopper, D. G., Pimm, M. V.: Radiation-killed BCG in the treatment of transplanted rat tumors. Int. J. Cancer 13, 743 (1974)

    Google Scholar 

  2. Bekierkunst, A., Wang, L., Toubiana, R., Lederer, E.: Immunotherapy of cancer with non-living BCG and fractions derived from mycobacteria: Role of cord factor (trehalose-6,6′-dimycolate) in tumor regression. Infect. Immunity 10, 1044 (1974)

    Google Scholar 

  3. Gray, G. R., Ribi, E., Granger, D., Parker, R., Azuma, I., Yamamoto, K.: Immunotherapy of cancer: Tumor suppression and regression by cell walls of Mycobacterium phlei attached to oil droplets. J. Natl. Cancer Inst. 55, 727 (1975)

    Google Scholar 

  4. Ribi, E. E., Granger, D. L., Milner, K. C., Strain, S. M.: Tumor regression caused by endotoxins and mycobacterial fractions. J. Natl. Cancer Inst. 55, 1253 (1975)

    Google Scholar 

  5. Yamamura, Y., Azuma, I., Taniyama, T., Sugimura, K., Hirao, F., Tokuzen, R., Okabe, M., Nakahara, W., Yasumoto, K.: Immunotherapy of cancer with cell wall skeleton of Mycobacterium bovis BCG. Annals N.Y. Acad. Science (in press)

  6. Zbar, B., Bernstein, I. D., Bartlett, G. L., Hanna, M. G., Jr., Rapp, H. J.: Immunotherapy of cancer: Regression of intradermal tumors and prevention of growth of lymph node metastases after intralesional injection of living Mycobacterium bovis. J. Natl. Cancer Inst. 49, 119 (1972)

    Google Scholar 

  7. Zbar, B., Rapp, H. J., Ribi, E. E.: Tumor suppression by cell walls of Mycobacterium bovis attached to oil droplets. J. Natl. Cancer Inst. 48, 831 (1972)

    Google Scholar 

  8. Zbar, B., Ribi, E., Meyer, T., Azuma, J., Rapp, H. J.: Immunotherapy of cancer: Regression of established intradermal tumors after intralesional injection of mycobacterial cell walls attached to oil droplets. J. Natl. Cancer Inst. 52, 1571 (1974)

    Google Scholar 

  9. Zbar, B., Ribi, E., Rapp, H. J.: An experimental model for immunotherapy of cancer. Natl. Cancer Inst. Monogr. 39, 3 (1973)

    Google Scholar 

  10. Zbar, B., Wepsic, H. T., Rapp, H. J., Whang-Peng, J., Borsos, T.: Transplantable hepatomas induced in strain 2 guinea pigs by diethylnitrosamine: Characterization by histology, growth and chromosomes. J. Natl. Cancer Inst. 43, 821 (1969)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Immunobiology, National Cancer Institute National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, 20014, Bethesda, Maryland, USA

    E. Yarkoni, H. J. Rapp & B. Zbar

Authors
  1. E. Yarkoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. J. Rapp
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Zbar
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yarkoni, E., Rapp, H.J. & Zbar, B. Immunotherapy of a guinea pig hepatoma with ultrasonically prepared mycobacterial vaccines. Cancer Immunol Immunother 2, 143–146 (1977). https://doi.org/10.1007/BF00200061

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00200061

Keywords

Search

Navigation