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Comparison of recombinant-interleukin-2-activated peripheral blood and tumor-infiltrating lymphocytes of patients with epithelial ovarian carcinoma: Cytotoxicity, growth kinetics and phenotype

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Summary

We have compared the growth and tumordirected cytotoxic efficacy of recombinant-interleukin-2-(rIL-2)-activated peripheral blood (PBL) and tumor-infiltrating lymphocytes (TIL) from patients with epithelial ovarian carcinoma. These studies demonstrated that TIL and PBL displayed similar levels of cytotoxicity and a broad range of target cell killing, as exemplified by their reactivity against autologous and allogeneic ovarian tumors as well as against tumor cell lines. No specificity of autologous tumor cell killing was manifested by TIL. Even though TIL of some patients showed higher proliferative activity (especially at the later times in rIL-2 culture) this was not a general phenomenon. In fact, in one case TIL did not proliferate at all, and in the other case the PBL proliferated more actively. While the cultures were composed primarily of CD3+ lymphocytes, the major cytotoxic cells displayed the CD56+ and CD16+ phenotype. Addition of OKT3 mAb to rIL-2 cultures resulted in an increased proliferative index, but showed only a minor effect on the cytotoxic potential of cultured lymphocytes. The therapeutic potential of rIL-2-activated TIL and PBL is discussed.

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Authors and Affiliations

  1. Section of Natural Immunity, Department of General Surgery, The University of Texas, M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 18, 77030, Houston, TX, USA

    Eva Lotzová (Professor of Immunology, Chief) & Cherylyn A. Savary

  2. Department of Gynecology, The University of Texas, M. D. Anderson Cancer Center, Houston, Texas, USA

    Ralph S. Freedman, Creighton L. Edwards & Mitchell Morris

Authors
  1. Eva Lotzová
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  2. Cherylyn A. Savary
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  3. Ralph S. Freedman
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  4. Creighton L. Edwards
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  5. Mitchell Morris
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Recipient of the Florence Maude Thomas Cancer Research Professorship

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Lotzová, E., Savary, C.A., Freedman, R.S. et al. Comparison of recombinant-interleukin-2-activated peripheral blood and tumor-infiltrating lymphocytes of patients with epithelial ovarian carcinoma: Cytotoxicity, growth kinetics and phenotype. Cancer Immunol Immunother 31, 169–175 (1990). https://doi.org/10.1007/BF01744732

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  • DOI: https://doi.org/10.1007/BF01744732

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