Summary
The lymphoblasts from 100 patients with acute lymphocytic leukaemia were investigated for the expression of receptors for sheep erythrocytes (E) and of a specific heterologous T cell antigen (T). In 17 cases, both T cell markers were expressed simultaneously on the leukaemic cells. In 13 cases only T antigens could be demonstrated on the lymphoblasts. A quantitative analysis of T antigens by immunoautoradiography revealed that the T expression of E−T+-lymphoblasts was in general like that of E+T+-lymphocytes in the blood of normal persons, in several cases even higher. Therefore, the failure of E-rosette formation cannot be correlated to a decrease of the other T cell differentiation marker. In 7 out of 9 tested cases, a strong acid phosphatase reaction product located paranuclearly could be demonstrated. Complement-receptors were expressed in 3 of 5 cases which were also demonstrated in some cases of the E+T+-ALL group. The latter group was characterized by a T antigen expression like that of thymocytes. 4 cases of the E−T+ALL group were adults. Since the leukaemia cells of 2 cases were negative for acid phosphatase, PAS and all surface markers including cALL antigen, the T antigen can classify undifferentiated and otherwise unclassificable leukaemias. The clinical significance of the E−T+-ALL seems to be important since 5 out of 9 children with this type of ALL died soon after diagnosis.
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Literatur
Aiuti, F., Wigzell, H.: Function and distribution of human lymphocytes. I. Production of anti-T lymphocyte sera as estimated by cytotoxicity and elimination of function of lymphocytes. Clin. Exp. Immunol.13, 171 (1973)
Barka, T., Anderson, P.J.: Histochemistry. Theory, Practice and Bibliography. New York, Evanston, London: Harper and Row 1965
Borella, L., Sen, L.: E receptors on blasts from untreated acute lymphocytic leukemia: Comparison of temperature dependence of E rosettes formed by normal and leukemic lymphoid cells. J. Immunol.114, 187 (1975)
Brouet, J.C., Valensi, F., Daniel, M.T., Flandrin, G., Preud'Homme, J.L., Seligmann, M.: Immunological classification of acute lymphoblastic leukemias: evaluation of its clinical significance in a hundred patients. Brit. J. Haemal.33, 319 (1976)
Dierich, M.P., Pellegrino, M.A., Ferrone, S., Reisfeld, R.A.: Evaluation of C3 receptors on lymphoid cells with different complement sources. J. Immunol.112, 1766 (1974)
Fu, S.M., Winchester, R.J., Kunkel, H.G.: The occurrence of the HL-B alloantigens on the cells of unclassified acute lymphoblastic leukemias. J. Exp. Med.142, 1334 (1975)
Gatien, J.G., Scheeberger, E.E., Merler, E.: Analysis of human thymocyte subpopulations using discontinuous gradients of albumin-precursor lymphocytes in human thymus. Euro. J. Immunol.5, 312 (1975)
Greaves, M.F., Brown, G., Rapson, N.T., Lister, T.A.: Antisera to acute lymphoblastic leukemia cells. Clin. Immunol. Immunopath.4, 67 (1975)
Jondal, M., Hohn, G., Wigzell, H.: Surface markers on human T and B lymphocytes. 1. A large population of lymphocytes forming non immune rosettes with sheep red blood cells. J. Exp. Med.136, 207 (1972)
Kersey, J.H., Sabad, A., Gajl-Peczalka, K., Hallgren, H.M., Yunis, E.J., Nesbit, M.E.: Acute lymphoblastic leukemic cells with T (thymus-derived) lymphocyte markers. Science182, 1355 (1973)
Lukes, R. J.: Slide seminar: Malign lymphoma. 10th International Congr. Int. Acad. Path., Hamburg 1974
Minowada, J., Ohnuma, T., Moore, G.E.: Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Nat. Cancer Inst.49, 891 (1972)
Minowada, J., Tsubota, T., Nakazawa, S. et al.: Establishment and characterization of leukemic T-cell lines, B-cell lines and Null-cell line: a progress report on surface antigen study of fresh lymphatic leukemias in man. In: Immunological Diagnosis of Leukemias and Lymphomas. Thierfelder, S., Rodt, H., Thiel, E. (eds.), p. 241. Berlin, Heidelberg, New York: Springer 1977
Rodt, H., Thierfelder, S., Thiel, E., Götze, D., Netzel, B., Huhn, D., Eulitz, M.: Identification and quantitation of human T-cell antigen by antisera purified from antibodies cross-reacting with hemopoietic progenitors and other blood cells. Immunogenetics2, 411 (1975)
Rodt, H., Netzel, B., Thiel, E., Jäger, G., Huhn, D., Haas, R., Götze, D., Thierfelder, S.: Classification of leukemic cells with T-and O-ALL specific antisera. In: Immunological Diagnosis of Leukemias and Lymphomas. Thierfelder, S., Rodt, H., Thiel, E. (eds.), p. 87. Berlin, Heidelberg, New York: Springer 1977
Sen, L., Borella, L.: Clinical importance of lymphoblasts with T markers in childhhod acute leukemia. N. Engl. J. Med.292, 828 (1975)
Smith, R.W., Terry, W.D., Buell, D.N., Sell, K.W.: An antigenic marker for human thymic lymphocytes. J. Immunol.110, 884 (1973)
Stein, H., Bouman, H., Lennert, K., Fuchs, J., Havsteen, B.: Isoelectric focusing pattern of IgM and surface markers in Non-Hodgkin's Lymphomas. In: Immunological Diagnosis of Leukemias and Lymphomas. Thierfelder, S., Rodt, H., Thiel, E. (eds.), p. 315. Berlin, Heidelberg, New York: Springer 1977
Thiel, E., Dörmer, P., Rodt, H., Thierfelder, S.: Quantitative immunoautoradiography at the cellular level. I. Design of a microphotometric method to quantitate membrane antigens on single cells using125I-labeled antibodies. J. Immunol. Methods2, 317 (1975)
Thiel, E., Dörmer, P., Eulitz, M.: Quantitative125J-Autoradiographie einzelner Zellen. Histochemistry43, 33 (1975)
Thiel, E., Rodt, H., Huhn, D., Thierfelder, S.: Decrease and altered distribution of human T antigen on chronic lymphatic leukemia cells of T type, suggesting a clonal origin. Blood47, 723 (1976)
Thiel, E., Dörmer, P., Rodt, H., Huhn, D., Bauchinger, M., Kley, H.P., Thierfelder, S.: Quantitation of T-antigenic sites and Ig-determinants on leukemic cells by microphotometric immunoautoradiography. Proof of the clonal origin of thymus-derived lymphocytic leukemias. In: Immunological Diagnosis of Leukemias and Lymphomas. Thierfelder, S., Rodt, H., Thiel, E. (eds.), p. 131. Berlin, Heidelberg, New York: Springer 1977
Tsukimoto, I., Wong, K.Y., Lampkin, B.C.: Surface markers and prognostic factors in acute lymphoblastic leukemia. N. Engl. J. Med.294, 245 (1976)
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Unterstützt von der Deutschen Forschungsgemeinschaft, Studie im Rahmen des Assoziations-vertrages Euratom/GSF Nr. 217-76-1 BIOD
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Thiel, E., Rodt, H., Netzel, B. et al. T-Zell-Antigen positive, E-Rosetten negative akute Lymphoblastenleukämie. Blut 36, 363–369 (1978). https://doi.org/10.1007/BF01000595
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DOI: https://doi.org/10.1007/BF01000595