Skip to main content
SpringerLink
Log in

Intestinal absorption of cobalt and iron: Mode of interaction and subcellular distribution

Intestinale Resorption von Kobalt und Eisen: Interaktion und subzelluläre Verteilung

  • Original Works
  • Published:
Blut Aims and scope Submit manuscript

Zusammenfassung

  1. 1.

    10 min nach Verabfolgung steigender Dosen59Fe-(FeCl3) und60Co-(CoCl2) (0,5-1000 nMol Metall) in abgebundene Duodenumschlingen normaler und eisenarmer Ratten zeigt die Resorptionskinetik für beide Metalle Sättigungscharakteristik; bei eisenarmen Ratten war die Resorption beider Metalle gesteigert.

  2. 2.

    Zusätze von Kobalt zu den59Fe-haltigen Testlösungen hemmten die Eisenresorption.

  3. 3.

    Die Untersuchung des zeitlichen Verlaufes dieser Interaktion an eisenarmen Ratten machte deutlich, da\ Kobalt die Abgabe von Eisen an das Blut hemmt; die Eisenaufnahme aus dem Lumen in die Mucosazellen war hingegen nicht betroffen.

  4. 4.

    Die subzelluläre Verteilung von59Fe und60Co in Mucosazellhomogenaten eisenarmer Ratten zeigt nach Ultrazentrifugation auf einer Polyvinylpyrrolidon-CsCl-Lösung ein ähnliches Verteilungsmuster für beide Metalle; in Anwesenheit von Kobalt ist die subzelluläre Verteilung von59Fe nicht geändert.

  5. 5.

    Aus diesen Befunden wird gefolgert, da\ Kobalt die Eisenresorption nicht infolge einer Interaktion mit eisenbindenden Liganden auf oder in der luminalen Membran der Mucosazellen hemmt, sondern da\ es mit Eisen bei der Abgabe aus dem Epithel ans Blut interferiert.

Summary

  1. 1.

    The absorption kinetic of59Fe-(FeCl3) and60Co-(CoCl2) 10 min after administration of increasing doses (0.5–1,000 nmoles metal) into tied-off duodenal segments of normal and iron-deficient rats shows saturation characteristic for both metals; in iron-deficient rats the absorption of both metals was enhanced.

  2. 2.

    The addition of increasing amounts of cobalt to the59Fe-containing test solutions caused a decrease of the absorption of iron.

  3. 3.

    The study of the time dependence of this interaction in iron-deficient rats revealed, that cobalt inhibits the release of iron from mucosal cells into the blood, whereas the uptake of iron from the lumen into the mucosal cells did not differ from the controls without administration of cobalt.

  4. 4.

    The subcellular distribution of59Fe and60Co in mucosal cell homogenates of iron-deficient rats after ultracentrifugation on a polyvinylpyrrolidone-CsCl solution shows a similar pattern for both metals; in the presence of cobalt the subcellular distribution of59Fe is not changed.

  5. 5.

    From these results the conclusion is drawn that cobalt inhibits iron absorption not by an interference with iron binding sites on or in the luminal membranes of the mucosal cells but by an interaction with the releasing process at the contraluminal side.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Bédard, Y.C., Pinkerton, P.H., Simon, G.T.: Radioautographic Observations on Iron Absorption by the Normal Mouse Duodenum. Blood38, 232–245 (1971)

    Google Scholar 

  2. Bédard, Y.C., Clarke, S., Pinkerton, P.H., Simon, G.T.: Effect of cycloheximide on iron absorption: “Toxicity of Iron”. Lab. Invest.30, 155–160 (1974)

    Google Scholar 

  3. Forth, W.: Untersuchungen über die Resorption von Eisen und chemisch verwandten Schwermetallen an den Därmen normaler und anämischer Ratten in vivo und in vitro; Ein Beitrag zur Frage der Spezifität des eisenbindenden Systems in der Mucosa. Habil. Schrift, Med. Fak., Homburg/Saar 1966

  4. Forth, W.: Resorption von Eisen und chemisch verwandten Metallen in vitro und in vivo; die Spezifität des eisenbindenden Systems in der Mucosa des Jejunums von Ratten. In: Frontiers of nucl. med. W. Horst (ed.), p. 83–103. Berlin, Heidelberg, New York: Springer 1971

    Google Scholar 

  5. Forth, W., Andres, H.: Methode zur Erzeugung eines Eisenmangels bei Ratten. Arzneim. Forsch.19, 363–364 (1969)

    Google Scholar 

  6. Forth, W., Rummel, W.: Iron Absorption. Physiol. Rev.53, 724–792 (1973)

    Google Scholar 

  7. Forth, W., Rummel, W., Becker, P. J., Andres, H.: Die vergleichende Prüfung von Bindung und Durchtritt von Eisen, Kobalt und Kupfer durch isolierte Jejunumsegmente normaler und anämischer Ratten. Med. Pharmacol. exp.15, 179–186 (1966)

    Google Scholar 

  8. Halliday, J.W., Powell, L.W.: The use of suspensions of isolated rat mucosal cells to study mechanisms of iron absorption. Clin. Chim. Acta43, 267–276 (1973)

    Google Scholar 

  9. Halliday, J.W., Powell, L.W., Mack, U.: Intestinal iron-bindung complexes in iron absorption. In: Proteins of Iron Storage and Transport in Biochemistry and Medicine. Crichton, R.R. (ed.), p. 405–410. Amsterdam: North Holland 1975

    Google Scholar 

  10. Halliday, J.W., Powell, L.W., Mack, U.: Iron absorption in the rat: The search for possible intestinal mucosal carriers. Br. J. Haematol.34, 237–250 (1976)

    Google Scholar 

  11. Huebers, H., Huebers, E., Forth, W., Rummel, W.: Binding of iron to a non-ferritin protein in the mucosal cells of normal and iron-deficient rats during absorption. Life Sci.10 (Part I), 1141–1148 (1971)

    Google Scholar 

  12. Huebers, H., Huebers, E., Forth, W., Rummel, W.: Iron absorption and iron-bindung proteins in the intestinal mucosa of mice with sex-linked anaemia. Hoppe-Seylers Z. Physiol. Chem.354, 1156–1158 (1973)

    Google Scholar 

  13. Huebers, H., Huebers, E., Rummel, W.: Dependence of increased iron absorption by irondeficient rats on an elutable component of jejunal mucosa. Hoppe-Seylers Z. Physiol. Chem.355, 1159–1161 (1974)

    Google Scholar 

  14. Huebers, H., Huebers, E., Rummel, W.: Mechanism of iron absorption: iron-binding proteins and dependence of iron absorption on an elutable factor. In: Iron Metabolism and its Disorders. Kief, H. (ed.), p. 13–24. Amsterdam: Excerpta Medica 1975

    Google Scholar 

  15. Huebers, H., Huebers, E., Rummel, W., Crichton, R.R.: Isolation and characterization from rat intestinal mucosa. Eur. J. Biochem.66, 447–455 (1976)

    Google Scholar 

  16. Huebers, H., Huebers, E., Simon, J., Forth, W.: A method for preparing stable density gradients and their applications for fractionation of intestinal mucosa cells. Life Sci.10 (Part II), 377–384 (1971)

    Google Scholar 

  17. Kaufman, N., Wyllie, J.C., Newkirk, M.: Two microsomal-associated iron-bindung proteins observed in rat small intestinal cells during iron absorption. Biochim. Biophys. Acta497, 719–727 (1977)

    Google Scholar 

  18. Morton, A.G., Tavill, A.S.: The role of iron in the regulation of hepatic transferrin synthesis. Br. J. Haematol.36, 383–394 (1977)

    Google Scholar 

  19. Pollack, S., George, J. N., Reba, R.C., Kaufman, R.M., Crosby, W.H.: The Absorption of Nonferrous Metals in Iron Deficiency. J. Clin. Invest.44, 1470–1473 (1965)

    Google Scholar 

  20. Rummel, W., Forth, W.: Zur Frage der metabolischen Abhängigkeit von Eisenbindung und Durchtritt durch den isolierten Dünndarm. Arch. Exptl. Pathol. Pharmakol.260, 50–57 (1968)

    Google Scholar 

  21. Schade, S.G., Felsher, B.F., Bernier, G.M., Conrad, M.E.: Interrelationship of cobalt and iron absorption. J. Lab. Clin. Med.75, 435–441 (1970)

    Google Scholar 

  22. Schade, S.G., Felsher, B.F., Glade, B.E., Conrad, M.E.: Effect of Cobalt upon Iron Absorption. Proc. Soc. Exp. Biol. Med.134, 741–743 (1970)

    Google Scholar 

  23. Sheehan, R.G.: Interrelatonships of Iron and Cobalt Absorption: Mucosal Distribution of Cobalt During Absorption. Proc. Soc. Exp. Biol. Med.146, 993–996 (1974)

    Google Scholar 

  24. Tan, A.T., Woodworth, R.C.: Ultraviolet Difference Spectral Studies of Conalbumin Complexes with Transition Metal Ions. Biochemistry8, 3711–3716 (1969)

    Google Scholar 

  25. Thomson, A.B.R., Shaver, C., Lee, D. J., Jones, L.B., Valberg, L.S.: Effect of varying iron stores on site of intestinal absorption of cobalt and iron. Am. J. Physiol.220, 674–678 (1971)

    Google Scholar 

  26. Thomson, A.B.R., Valberg, L.S.: Kinetics of intestinal iron absorption in the rat: effect of cobalt. Am. J. Physiol.220, 1080–1085 (1971)

    Google Scholar 

  27. Thomson, A.B.R., Valberg, L.S.: Intestinal uptake of iron, cobalt and manganese in the iron-deficient rat. Am. J. Physiol.223, 1327–1329 (1972)

    Google Scholar 

  28. Thomson, A.B.R., Valberg, L.S.: Kinetics of the Subcellular Distribution of Iron and Cobalt in the Intestinal Mucosa of the Rat. Dig. Dis.21, 305–312 (1976)

    Google Scholar 

  29. Thomson, A.B.R., Valberg, L.S., Sinclair, D.G.: Competitive Nature of the Intestinal Transport Mechanism for Cobalt and Iron in the Rat. J. Clin. Invest.50, 2384–2394 (1971)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie der Universität des Saarlandes, D-6650, Homburg/Saar, Germany

    G. Becker, H. Huebers & W. Rummel

Authors
  1. G. Becker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Huebers
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. Rummel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Becker, G., Huebers, H. & Rummel, W. Intestinal absorption of cobalt and iron: Mode of interaction and subcellular distribution. Blut 38, 397–406 (1979). https://doi.org/10.1007/BF01007901

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01007901

Schlüsselwörter

Key words

Search

Navigation