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Purine metabolism in human lymphocytes

Purinstoffwechsel in menschlichen Lymphozyten

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Zusammenfassung

In peripheren menschlichen Lymphozyten wurden die Aufnahme und der Stoffwechsel von Adenin, Guanin und Hypoxanthin untersucht. Die gereinigten Lymphozyten wurden mit14C-markierten Purinbasen inkubiert und anschlie\end vom Inkubationsmedium mittels rascher Filtrationstechnik getrennt. Eine bevorzugte Aufnahme einer der Purinbasen konnte nicht beobachtet werden. Die dünnschichtchromatographische Auftrennung der säurelöslichen Metabolite ergab kaum eine Bildung von Nukleosiden und anderen als den zugesetzten Purinbasen. Adenin wurde zu ca. 2/3, Guanin und Hypoxanthin hingegen zu etwa der Hälfte zu Nukleotiden aufgebaut, wobei Adenin und Guanin hauptsächlich zu ihren eigenen, Hypoxanthin jedoch zu Adeninnukiektiden metabolisiert wurde. Die Ergebnisse weisen auf die bevorzugte Bildung von Adeninnukleotiden in normalen menschlichen Lymphozyten.

Summary

In peripheral human blood lymphocytes the uptake and metabolism of adenine, guanine, and hypoxanthine was investigated. This was achieved by incubation of purified lymphocytes with14C-purine bases, separation of cells from the incubation medium by a rapid filtration technique, and subsequent separation of the acid soluble material by thin-layer chromatography. No preferential uptake for one of the purine bases was observed. In all cases only traces of14C-purine bases not added originally and labeled nucleosides could be demonstrated. Approximately 2/3 of adenine and 1/2 of guanine or hypoxanthine were converted to nucleotides. Separation of formed nucleotides showed that adenine and guanine were metabolized mainly to their corresponding nucleotides; hypoxanthine was converted to a considerable amount to adenine nucleotides and only to a small proportion into its own nucleotides. These results demonstrate the predominance of adenine nucleotide formation in normal human lymphocytes.

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Authors and Affiliations

  1. 2. Medizinische Universitätsklinik Wien, Garnisongasse 13, A-1090, Wien, Österreich

    M. M. Müller

  2. Institut für Medizinische Chemie der Universität Wien, Wien, Österreich

    G. Pischek

  3. Institut für Allgemeine und Experimentelle Pathologie der Universität Wien, Wien, Österreich

    O. Scheiner

  4. Institut für Spezifische Prophylaxe und Tropenmedizin der Universität Wien, Wien, Österreich

    H. Stemberger & G. Wiedermann

Authors
  1. M. M. Müller
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  2. G. Pischek
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  3. O. Scheiner
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  4. H. Stemberger
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  5. G. Wiedermann
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Additional information

The study was supported by a grant of “Fonds zur Förderung der wissenschaftlichen Forschung Österreichs” (project No. 3038)

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Müller, M.M., Pischek, G., Scheiner, O. et al. Purine metabolism in human lymphocytes. Blut 38, 447–455 (1979). https://doi.org/10.1007/BF01013505

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  • DOI: https://doi.org/10.1007/BF01013505

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