Zusammenfassung
Zwei hämatologisch normale Patienten mit Glioblastoma multiforme und sechs Patienten mit chronischer lymphatischer Leukämie (CLL) erhielten3H-Thymidin-Infusionen von 3 bis 10 Tagen Dauer. In autoradiographierten Blutausstrichen wurden der Markierungsindex und die Markierungsintensität der Granulozyten bestimmt. Um optimale Markierungsverhältnisse zu erreichen, waren sehr lange autoradiographische Expositionszeiten erforderlich. Die mathematische Analyse der Markierungsindex-Kurven ermöglichte die Schätzung folgender kinetischer Parameter der postmitotischen
Granulozyten: mittlere Reifungs- und Speicherungszeit im Knochenmark (115 ± 7 Std. für Neutrophile, 103 ± 4 Std. für Eosinophile und 103 ± 11 Std. für Basophile); mittlere relative Einstromrate ins Blut (4,2 ± 0,4%/Std. für Neutrophile, 4,0 ± 0,4%/Std. für Eosinophile und 1,2 ± 0,3%/Std. für Basophile); mittlere Blut-Transitzeit (25 ± 2 Std. für Neutrophile, 26 ± 3 Std. für Eosinophile und 89 ± 21 Std. für Basophile); mittlere Halbwertzeit im Blut (17,3 ± 1,4 Std. für Neutrophile, 18,0 ± 2,1 Std. für Eosinophile und 62 ± 15 Std. für Basophile).
Unter den vorliegenden Bedingungen schien die Kinetik der Granulozyten bei den untersuchten CLL-Patienten trotz erheblicher lymphozytärer Infiltration des Knochenmarks normal zu sein.
Der auffällige Unterschied der Bluthalbwertszeit für Neutrophile in dieser Studie und den früher publiziertem Werte aufgrund von Autotransfusionen DFP-markierter Granulozyten wird diskutiert.
Summary
Two hematologically normal patients with glioblastoma and six patients with chronic lymphocytic leukemia received continuous3H-thymidine infusions for 3–10 days. In autoradiographs of blood cell smears taken for 25 days or more after the beginning of3H-thymidine administration the labeling index and the labeling intensity of granulocytes were determined. A sufficiently high labeling intensity, i. e. a sufficiently long autoradiographic exposure time was found to be critical for obtaining valid and reproducible results. On the basis of certain assumptions discussed in detail, complete labeling of cells with3H-thymidine followed by autoradiographic evaluation and mathematical analysis of the labeling patterns seems to be a suitable method for estimation of kinetic parameters of postmitotic granulocytes in vivo.
The mean intramedullary maturation and storage time was observed to be 115 ± 7 h for neutrophils, 103 ± 4 h for eosinophils and 103 ± 11 h for basophils. The mean relative inflow rate into the blood (or relative turnover rate in the blood) was found to be 4.2 ± 0.4 %/h for neutrophils, 4.0 ± 0.4 %/h for eosinophils and 1.2 ± 0.3 %/h for basophils. The mean blood transit time (or blood sojourn time) was estimated to be 25 ± 2 h for neutrophils, 26 ± 3 h for eosinophils and 89 ± 21 h for basophils. Accordingly the half lifes (T 1/2) of granulocytes in the blood were 17.3 ± 1.4 h for neutrophils, 18.0 ± 2.1 foi eosinophils and 62 ± 15 h for basophils. Under the quasi steady state conditions of this study the kinetics of granulocytes in the present CLL patients appeared to be normal, despite a marked lymphocytic infiltration of the bone marrow.
The apparent discrepancy between these findings and the data obtained with autotransfusion of DFP-labeled granulocytes is discussed.
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Supported by the Deutsche Forschungsgemeinschaft (SFB 112 and Be 79/14)
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Steinbach, K.H., Schick, P., Trepel, F. et al. Estimation of kinetic parameters of neutrophilic, eosinophilic, and basophilic granulocytes in human blood. Blut 39, 27–38 (1979). https://doi.org/10.1007/BF01008072
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DOI: https://doi.org/10.1007/BF01008072