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Demonstration of antibodies binding to autologous and allogeneic leukemic cells in childhood ALL

Evidence for a common ALL antigen(s)

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Summary

The humoral immune response to autologous leukemic cells was investigated in childhood ALL using a 125I protein A binding assay. In 5/7 patients antibodies were demonstrated at diagnosis and in 3/7 cases also after chemotherapy. Sera from 2/3 patients, which bound significantly to autologous leukemic cells, did not bind significantly to autologous remission cells. In allogeneic experiments sera bound significantly to ALL leukemic cells (6/7 positive combinations), but not to AML leukemic cells (8/8 negative combinations). We propose that ALL sera contain antibodies binding to autologous leukemic cells and that they are directed against a common ALL antigen(s).

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Abbreviations

ALL:

acute lymphoblastic leukemia

AML:

acute myeloid leukemia

CALLA:

common ALL antigen

CPM:

counts per minute

HTLV 1:

human T cell leukemia/lymphoma virus, type 1

I a:

immune-associated antigens

SE:

standard error

References

  1. Ankerst J, Fäldt R, Nilsson PG, Flodgren P, Sjögren HO (1984) Complete remission in a patient with acute myelogenous leukemia treated with leukocyte alpha interferon and cimetidine. Cancer Immunol Immunother 17: 69–71

    Google Scholar 

  2. Baker MA, Falk JA, Carter WH, Taub RN (1979) The Toronto Leukemia Study Group: Early diagnosis of relapse in acute myelogenous leukemia: Serologic detection of leukemia-associated antigens in human marrow. N Engl J Med 301: 1353–1357

    Google Scholar 

  3. Bertini M, Galetto G, Comoglio PM (1982) Immune complexes and circulating antibodies against autologous leukemic cells in patients with acute leukemias. Immunology 45: 569–575

    Google Scholar 

  4. Blomberg J, Fäldt R (1985) Antibodies to human adult T cell leukemia virus type 1 associated antigens in Swedish leukemia patients and blood donors. Br J Haematol 60: 555–557

    Google Scholar 

  5. Dreesman GR, Brill DL, Fernbach DJ, Benyesh-Melnick M (1973) Cytotoxic antibody to autologous lymphocytes in childhood leukemia. Proc Am Assoc Cancer Res 14: 85

    Google Scholar 

  6. Fäldt R, Ankerst J (1977) Tumor-associated humoral cytotoxicity in patients with acute myelogenous leukemia before and after chemotherapy. Int J Cancer 20: 824–833

    Google Scholar 

  7. Fäldt R, Ankerst J (1979) Demonstration of antibody-associated cellular cytotoxicity in patients with acute myelogenous leukemia before and after chemotherapy. Int J Cancer 24: 17–26

    Google Scholar 

  8. Fäldt R, Ankerst J (1983) A 125I protein A binding assay detecting antibodies to cell surface antigens: Evidence for the presence of specific antibodies against leukemia-associated antigens in human leukemias. Cancer Immunol Immunother 15: 69–77

    Google Scholar 

  9. Greaves MF, Brown G, Rapson MT, Lister TA (1975) Antisera to acute lymphoblastic leukemia cells. Clin Immunol Immunopathol 4: 67–84

    Google Scholar 

  10. Langone JJ, Boyle MDP, Borsos T (1977) 125I protein A. Applications to the quantitative determination of fluid phase and cell-bound IgG. J Immunol Methods 18: 281–293

    Google Scholar 

  11. Laurell CB (1972) Electroimmunoassay. Scand J Clin Lab Invest 29, Suppl 124: 21–37

  12. Metzgar RS, Mohanakumar T, Milles DS (1972) Antigens specific for human lymphocytic and myeloid leukemia cells. Science 1781: 986–988

    Google Scholar 

  13. Mitchell MS, Birnbaum Mokyr M, Aspnes GT, McIntosh S (1973) Cytophilic antibodies in man. Ann Internat Med 79: 333–339

    Google Scholar 

  14. Negoro S, Seon BK (1981) Strong, specific anti-human leukemia antisera prepared with the use of purified cell membrane antigen. Cancer Res 41: 2973–2976

    Google Scholar 

  15. Ritz J, Sallan SE, Bast RC, Lipton JM, Nathan DG, Schlossman SF (1981) Autologous bone marrow transplantation in cALLA positive ALL following in vitro treatment with J 5 monoclonal antibody and complement. Blood 58 (suppl 1): 175a

  16. Robert-Guroff M, Ruscetti FW, Posner LE, Poiesz BJ, Gallo RC (1981) Detection of the human T cell lymphoma virus p19 in cells of some patients with cutaneous T cell lymphoma and leukemia using a monoclonal antibody. J Exp Med 154: 1957–1964

    Google Scholar 

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Authors and Affiliations

  1. Dept. of Internal Medicine, University of Lund, S-22185, Lund, Sweden

    R. Fäldt & J. Ankerst

  2. Dept. of Pediatrics, University of Lund, S-22185, Lund, Sweden

    S. Garwicz

  3. the Wallenberg Laboratory, University of Lund, S-22185, Lund, Sweden

    R. Fäldt & J. Ankerst

Authors
  1. R. Fäldt
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  2. J. Ankerst
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  3. S. Garwicz
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Fäldt, R., Ankerst, J. & Garwicz, S. Demonstration of antibodies binding to autologous and allogeneic leukemic cells in childhood ALL. Blut 52, 337–343 (1986). https://doi.org/10.1007/BF00320780

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  • DOI: https://doi.org/10.1007/BF00320780

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