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Multi-chemothermoimmunotherapy for human colon adenocarcinoma in vitro

  • Chemotherapy, TNF, Hyperthermia, Colon Adenocarcinoma, Multimodality
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Abstract

Effective adjunctive therapies for colorectal carcinoma are clearly needed. We evaluated the cytotoxic responses in vitro of human colon carcinoma cell lines to combined modalities: 5-fluorouracil/leucovorin (5-FU/LV), carboplatin (CP), tumor necrosis factor (TNF) and hyperthermia (HTX). Cytotoxicity was evaluated in a cell proliferation assay using crystal violet staining. 5-FU/LV was administered 2–3 days before TNF and CP, followed 1 h later by HTX. These cell lines were relatively resistant to HTX alone (42°C for 2 h), but were heterogeneous in their responses to various doses of the other single agents. This heterogeneity was also evident for combined modalities: the geneity was also evident for combined modalities: the HCT-15 cell line exhibited significant supra-additivity for selected doses of CP, TNF and 5-FU/LV, which was further enhanced by hyperthermia. In contrast, the HT-29 cell line did not demonstrate a strong pattern for supra-additivity, whereas the DLD-1 cell line had an intermediate response. Thus, our results suggest one approach to develop effective and dose-sparing multimodality therapeutic regimens for colon adenocarcinoma.

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Authors and Affiliations

  1. Department of Tumor Biology, University of Texas, Houston, Texas, USA

    Jim Klostergaard, M. Elena Leroux & Stephen P. Tomasovi

  2. Department of Clinical Investigation, University of Texas, Houston, Texas, USA

    Zahid H. Siddik

  3. Department of Biometry, University of Texas, Houston, Texas, USA

    H. -A. Hsu & Bartholomew P. Hsi

  4. Department of Internal Medicine, University of Texas, Houston, Texas, USA

    Lynn L. Danhauser

Authors
  1. Jim Klostergaard
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  2. M. Elena Leroux
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  3. H. -A. Hsu
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  4. Bartholomew P. Hsi
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  5. Zahid H. Siddik
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  6. Lynn L. Danhauser
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  7. Stephen P. Tomasovi
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Klostergaard, J., Leroux, M.E., Hsu, H.A. et al. Multi-chemothermoimmunotherapy for human colon adenocarcinoma in vitro. Cancer Chemother. Pharmacol. 37, 235–241 (1995). https://doi.org/10.1007/BF00688322

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  • DOI: https://doi.org/10.1007/BF00688322

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