Skip to main content
SpringerLink
Log in

Characterization of B-cell lines from SLE patients and their relatives

  • Original Article
  • Published:
Rheumatology International Aims and scope Submit manuscript

Abstract

Epstein-Barr-virus (EBV)-transformed lymphoblastoid B-cell lines were generated from peripheral blood lymphocytes of 55 patients with systemic lupus erythematosus (SLE) and 44 healthy relatives. All donors have previously been extensively characterized with regard to clinical, serologic, and genetic parameters. Here, peripheral blood lymphocytes and lines were characterized for cell surface antigens. Furthermore, autoantibody production and proliferation rate of the cell lines were monitored. A significant difference between patients and relatives was the lower proliferation rate of EBV-transformed cell lines of the SLE patients. All SLE cell lines are available for interested researches and can be obtained from the European Cell Bank, Salisbury, UK.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Klinman DM, Shirai A, Ishigatsubo Y, Conover J, Steinberg AD (1991) Quantitation of IgM-and IgG-secreting B cells in the peripheral blood of patients with systemic lupus erythematosus. Arthritis Rheum 34:1404–1410

    Google Scholar 

  2. Tanaka Y, Shirikawa F, Ota T, Suzuki H, Eto S, Yamashita U (1991) Mechanism of spontaneous activation of B cells in patients with systemic lupus erythematosus. Analysis with anticlass II antibody. J Immunol 140:761–767

    Google Scholar 

  3. Ogawa N, Itoh M, Goto Y (1992) Abnormal production of B-cell growth factor in patients with systemic lupus erythematosus. Clin Exp Immunol 89:26–31

    Google Scholar 

  4. Tan EM (1989) Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol 44:93–151

    Google Scholar 

  5. Arnett FC, Reveille JD (1992) Genetics of systemic lupus erythematosus. Rheum Dis Clin North Am 18:865–892

    Google Scholar 

  6. Hartung K, Baur MP, Coldewey R, Kalden JR, Lakomek HJ, Peter HH, Schendel D, Schneider PM, Seuchter SA, Stangel W, Deicher HRG, the Members of the Multicenter Study Group (1992) MHC-haplotypes and complement C4-alleles in systemic lupus erythematosus-results of a multicenter study. J Clin Invest 90:1346–1351

    Google Scholar 

  7. Bettinotti MP, Hartung K, Deicher HR, Keller E, Mikschl S, Albert E (1993) DR 2 haplotypes (DRB 1, DQA 1, DQB 1) associated with systemic lupus erythematosus. Immunogenetics 38:74–77

    Google Scholar 

  8. Hartung K, Renz M, Ehrfeld H, Deicher H, Seelig HP (1994) Genetische Assoziationen von Autoantikörpern gegen rekombinante Antigene der ENA-Gruppe und Cardiolipin beim systemischen Lupus erythematodes (SLE). Z Arztl Fortbild 88:491–494

    Google Scholar 

  9. Tan EM, Cohen AS, Fries FS, Masi AT, McShane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ (1982) The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 25:1271–1277

    Google Scholar 

  10. Boyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 21 [Suppl 97]:77–89

    Google Scholar 

  11. Wertel T, Uciechowski P, Schreiber C, Tetteroo PAT, Neeleman AP, Kurrle R, Deicher H, Schmidt RE (1989) Activation of cloned natural killer cells via FcR III. J Immunol 142:1102–1106

    Google Scholar 

  12. Schmidt RE, MacDermott RP, Bartley GT, Bertovich M, Amato DA, Austen KF, Schlossman SF, Stevens RL, Ritz J (1985) Specific release of proteoglycans from human natural killer cells during target cell lysis. Nature 318:289–291

    Google Scholar 

  13. Knapp W, Dörken B, Gills WR, Rieber EP, Schmidt RE, Stein H, van dem Borne AEGKr (1989) Leucocyte differentiation antigens IV. Oxford University Press, Oxford

    Google Scholar 

  14. Freyer DR, Morganroth ML, Rogers CE, Arnaout MA, Todd RF (1988) Modulation of surface Cd 11/CD 18 glycoproteins (Mo 1, LFA-1, p150,95) by human mononuclear phagocytes. Clin Immunol Immunopathol 46:272–283

    Google Scholar 

  15. Ostendorf T, Nishan C, Schubert J, Grussenmeyer T, Scholz C, Zielinska-Skowronek M, Schmidt RE (1995) Heterogeneous PIG-A mutations in different cell lineages in paroxysmal nocturnal hemoglobinuria. Blood (in press)

  16. Miller G, Lipman M (1973) Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Proc Natl Acad Sci USA 70:190–194

    Google Scholar 

  17. Amigorena S, Bonnerot C, Drake JR, Choquet D, Hunziker W, Guillet JG, Webster P, Sautes C, Mellman I, Fridman WH (1992) Cytoplasmic domain heterogeneity and functions of IgG Fc receptors in B lymphocytes. Science 256:1808–1812

    Google Scholar 

  18. Brooks DG, Qiu WO, Luster AD, Ravetch JR (1989) Structure and expression of human IgG FcR II (CD 32). J Exp Med 170:1369–1385

    Google Scholar 

  19. Amigorena S, Bonnerot C, Choquet D, Fridman WH, Teillaud JL (1989) FcR II expression in resting and activated B lymphocytes. Eur J Immunol 19:1379–1385

    Google Scholar 

  20. Witte T, Dumoulin FL, Geßner JE, Schubert J, Götze O, Neumann C, Todd RF III, Deicher H, Schmidt RE (1993) Defect of a complement receptor 3 epitope in a patient with systemic lupus erythematosus. J Clin Invest 92:1181–1187

    Google Scholar 

  21. Bende RJ, Jochems GJ, Frame TH, Klein MR, van Eijk RV, van Lier RA, Zeijlemaker WR (1992) Effects of IL-4, IL-5, and IL-6 on growth and immunoglobulin production of Epstein-Barr virus transformed cells. Cell Immunol 143:310–323

    Google Scholar 

  22. Schwab J, Lukowsky A, Volk HD, Peter HH, Melchers I (1994) Precursor frequencies for DNA-specific B-lymphocytes in patients with systemic lupus erythematosus (SLE). Clin Exp Immunol 96:450–457

    Google Scholar 

  23. Avila-Carino J, Torsteinsdottir S, Ehlin-Henriksson B, Masucci MG, Klein E (1991) Search for the critical characteristics of phenotypically different B cell lines, Burkitt lymphoma cells and lymphoblastoid cell lines, which determine differences in their functional interaction with allogeneic lymphocytes. Cancer Immunol Immunother 34:128–132

    Google Scholar 

  24. Ravetch JV, Kinet JP (1991) Fc receptors. Annu Rev Immunol 9:457

    Google Scholar 

  25. Gosselin EJ, Brown MF, Anderson CL, Zipf TF, Guyre PM (1990) The monoclonal antibody 41 H 16 detects the Leu 4 responder form of human Fc gamma RII. J Immunol 144:1817–1822

    Google Scholar 

  26. Salmon JE, Edberg JC, Brogle NL, Kimberly RP (1992) Allelic polymorphisms of human Fc gamma receptor II A and Fc gamma receptor IIIB. Independent mechanisms for differences in human phagocytic function. J Clin Invest 89:1274–1281

    Google Scholar 

  27. Duits AJ, Bootsma H, Spronk P, Gmelig-Meyling FHJ, Derksen R, Kater L, Capel PJA, van de Winkel JGJ (1993) Altered distribution of IgG Fc receptor IIa (CD 32) polymorphism in SLE patients. Abstract 6 in Second European Conference on systemic lupus erythematosus

  28. Engelhardt W, Matzke J, Schmidt RE (1995) Activation dependent expression of low affinity IgG receptors FcγRII (CD 32) and FcγRIII (CD 16) in subpopulations of human T lymphocytes. (in press)

  29. Von Wussow P, Jakschies D, Hochkeppel H, Horisberger M, Hartung K, Deicher H (1989) MX homologous protein in mononuclear cells from patients with systemic lupus erythematosus. Arthritis Rheum 32:914–918

    Google Scholar 

Download references

Author information

Author notes

  1. T. Witte

    Present address: Abteilung Klinische Immunologie, Zentrum Innere Medizin und Dermatologie, Medizinische Hochschule, Hannover, Germany

Authors and Affiliations

  1. Dana-Farber Cancer Institute Division of Immunology, Harvard Medical School, 44 Binney St., 02115, Boston, MA, USA

    T. Witte

  2. Abteilung Klinische Immunologic, Medizinische Hochschule, Hannover, Germany

    F. M. Bode, R. E. Schmidt & H. Deicher

  3. Zentallabor, Zentralkrankenhaus Reinkenheide, Bremerhaven, Germany

    K. Hartung

Authors
  1. T. Witte
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Hartung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. M. Bode
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. E. Schmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Deicher
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Witte, T., Hartung, K., Bode, F.M. et al. Characterization of B-cell lines from SLE patients and their relatives. Rheumatol Int 15, 89–93 (1995). https://doi.org/10.1007/BF00302123

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00302123

Key words

Search

Navigation