Summary
In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. Selection for organogenic tissue resulted in cultures yielding approximately three times more petioles than random subcultures. Callus-derived shoots could be rooted and established in the greenhouse. The method allowed for the production of thousands of plants but the regenerants often showed deviant phenotypes and genotypes.
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Abbreviations
- BA:
-
6-benzylaminopurine
- BMP:
-
basal medium propagation
- BMR:
-
basal medium rooting
- DAPI:
-
4′,6-diamino-2-phenylindole
- KIBA:
-
potassium salt of indole-3-butyric acid
- kinetin:
-
6-furfurylaminopurine
- MS:
-
Murashige and Skoog
- NAA:
-
1-naphthaleneacetic acid
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Communicated by K. Glimelius
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Dillen, W., Dijkstra, I. & Oud, J. Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill.. Plant Cell Reports 15, 545–548 (1996). https://doi.org/10.1007/BF00232991
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DOI: https://doi.org/10.1007/BF00232991