Abstract
Expression of Panicum miliaceum L. (proso millet) mitochondrial and cytosolic aspartate aminotransferase (mAspAT and cAspAT, respectively) genes in transgenic tobacco plants (Nicotiana tabacum) and their influences on protein synthesis were examined. The mAspAT- or cAspAT-transformed plants had about threefold or 3.5-fold higher AspAT activity in the leaf than non-transformed plants, respectively. Interestingly, the leaves of both transformed plants had increased levels of phosphoenolpyruvate carboxylase (PEPC) and transformed plants with cAspAT also had increased levels of mAspAT in the leaf. These results suggest that the increased expression of Panicum cAspAT in transgenic tobacco enhances the expression of its endogenous mAspAT and PEPC, and the increased expression of Panicum mAspAT enhances the expression of its endogenous PEPC.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 6 February 1998 / Revision received: 6 August 1999 · Accepted: 6 September 1999
Rights and permissions
About this article
Cite this article
Sentoku, N., Taniguchi, M., Sugiyama, T. et al. Analysis of the transgenic tobacco plants expressing Panicum miliaceum aspartate aminotransferase genes. Plant Cell Reports 19, 598–603 (2000). https://doi.org/10.1007/s002990050779
Issue Date:
DOI: https://doi.org/10.1007/s002990050779