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RLCC-isolation of Raucaffricine from its most efficient source — cell suspension cultures of Rauwolfia serpentina Benth

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Abstract

Raucaffricine (vomilenine-galactoside) was shown to be the major indole alkaloid of Rauwolfia serpentina Benth. cell suspension cultures grown in AP-medium (alkaloid production medium). Several grams of this glycoalkaloid can conveniently be isolated by RLCC (Rotation Locular Countercurrent Chromatography). A newly discovered enzyme efficiently converts the glycoalkaloid to its aglycon, vomilenine, which occupies a key function in the biosynthesis of ajmaline. This is the first demonstration of the occurrence of raucaffricine in Rauwolfia serpentina.

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Authors and Affiliations

  1. Lehrstuhl Pharmazeutische Biologie, Ludwig-Maximilians-Universität, Karlstr. 29, D-8000, München, Federal Republic of Germany

    Helmut Schübel & Joachim Stöckigt

Authors
  1. Helmut Schübel
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  2. Joachim Stöckigt
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Communicated by M. H. Zenk

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Schübel, H., Stöckigt, J. RLCC-isolation of Raucaffricine from its most efficient source — cell suspension cultures of Rauwolfia serpentina Benth. Plant Cell Reports 3, 72–74 (1984). https://doi.org/10.1007/BF00270975

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  • DOI: https://doi.org/10.1007/BF00270975

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