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Biochemical characterisation of Nicotiana plumbaginifolia auxotrophs that require branched-chain amino acids

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Abstract

The biochemical lesions in six amino acid-requiring auxotrophic lines of Nicotiana plumbaginifolia have been investigated, by means of feeding experiments with [14C] and unlabelled substrates, and enzyme analysis. Three of the lines require isoleucine for growth, are unable to synthesise 2-oxobutyrate in vivo and have no detectable threonine dehydratase (E.C.4.2.1.16) in vitro. The other three lines require (isoleucine + valine), accumulate [14C] dihydroxymethylvalerate when fed [14C]-L-threonine, and contain no detectable dihydroxyacid dehydratase (E.C.4.2.1.9).

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Authors and Affiliations

  1. Biochemistry Dept., Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK

    Roger M. Wallsgrove, Ruth Risiott & Simon W. J. Bright

  2. Plantengenetica, Institute of Molecular Biology, B-1640, St. Genesius Rode, Belgium

    Ioan Negrutiu

Authors
  1. Roger M. Wallsgrove
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  2. Ruth Risiott
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  3. Ioan Negrutiu
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  4. Simon W. J. Bright
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Communicated by P. J. King

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Wallsgrove, R.M., Risiott, R., Negrutiu, I. et al. Biochemical characterisation of Nicotiana plumbaginifolia auxotrophs that require branched-chain amino acids. Plant Cell Reports 5, 223–226 (1986). https://doi.org/10.1007/BF00269125

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  • DOI: https://doi.org/10.1007/BF00269125

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