Abstract
The production of pantothenic acid and thiamine by Azotobacter vinelandii was studied in a chemically defined medium and a dialysed soil medium amended with different amounts of carbohydrate and NH sup+inf4 . Pantothenic acid and thiamine were produced in large amounts in the culture medium containing 2.0–5.0% glucose and 0.1–0.3% NH4Cl. The results showed that the liberation of these vitamins was increased under adiazotrophic conditions and with an excess of C source.
Group of Environmental Microbiology, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, 18071 Granada, Spain.
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References
Barea JM, Brown ME (1974) Effect of plant growth produced by Azotobacter paspali related to synthesis of plant growth regulating substances. J Appl Bacteriol 37: 583–593
Brown ME (1982) Nitrogen fixation by free-living bacteria associated with plants-fact or fiction? In: Rhodes-Roberts ME, Skinner FA (eds) Bacteria and plants. Academic Press, London, pp 25–41
Döbereiner J, Pedrosa FO (1987) Nitrogen-fixing bacteria in nonleguminous crops plants. Brock Springer Series in Contemporary Bioscience Sci Tech Publ, Madison, Wis
Gonzalez-Lopez J, Salmeron V, Moreno J, Ramos-Cormenzana A (1983) Amino acids and vitamins produced by Azotobacter venelandii ATCC 12837 in chemically-defined media and dialysed soil media. Soil Biol Biochem 6: 711–713
Gonzalez-Lopez J, Salmeron V, Martinez-Toledo MV, Ballesteros F, Ramos-Cormenzana A (1986) Production of auxins, gibberellins and cytokinins by Azotobacter vinelandii ATCC 12837 in chemically-defined media and dialysed soil media. Soil Biol Biochem 18: 119–120
Gonzalez-Lopez J, Martinez-Toledo MV, Reina S, Salmeron V (1991) Root exudates of maize and production of auxins, gibberellins, cytokinins, amino acids and vitamins by Azotobacter chroococcum in chemically-defined media and dialysed-soil media. Toxicol Environ Chem 33: 69–78
Hussain A, Arshad M, Hussain A, Hussain F (1987) Response of maize (Zea mays) to Azotobacter inoculation under fertilized and unfertilized conditions. Biol Fertil Soils. 4: 73–77
Martinez-Toledo MV, Gonzalez-Lopez J, de laRubia T, Ramos-Cormenzana A (1985) Isolation and characterization of Azotobacter chroococcum from the roots of Zea mays. FEMS Microbiol Ecol 31: 197–203
Martinez-Toledo MV, Salmeron V, Gonzalez-Lopez J (1991) Biological characteristics of Azotobacter spp. in natural environments. Trends Soil Sci 1: 15–23
Okon Y (1984) The physiology of Azospirillum in relation to its utilization as inoculum for promoting growth in plants. In: Ludden LW, Burris IE (eds) Proceedings of the Fourteenth Steinbock Symposium at the University of Wisconsin. Elsevier Science Publishing, New York, pp 165–174
Rodelas B, Salmeron V, Martinez-Toledo MV, Gonzalez-Lopez J (1993) Production of vitamins by Azospirillum brasilense in chemically-defined media. Plant and Soil 153: 97–101
Vela GR, Rosenthal RS (1972) Effect of peptone on Azotobacter morphology. J Bacteriol 111: 260–266
Zimmer W, Roeben K, Bothe H (1988) An alternative explanation for plant growth promotion by bacteria of the genus Azospirillum. Planta 176: 333–342
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Martinez-Toledo, M.V., Rodelas, B., Salmeron, V. et al. Production of pantothenic acid and thiamine by Azotobacter vinelandii in a chemically defined medium and a dialysed soil medium. Biol Fert Soils 22, 131–135 (1996). https://doi.org/10.1007/BF00384444
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DOI: https://doi.org/10.1007/BF00384444