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Intraneural injection of lymphocytes in experimental allergic neuritis

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Summary

Passive transfer of experimental allergic neuritis (EAN) lymph node cells (LNC) by intraneural injection did not produce significant demyelination. EAN-LNC stimulated with myelin in vitro produced mild demyelination while those incubated with Concanavalin A had no effect. The lack of demyelination by unstimulated EAN-LNC is in contrast to the marked demyelination produced by intraneural injection of EAN serum. The mononuclear cell infiltration and demyelination of classical EAN seem to require both cellular and humoral immune responses.

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References

  • Abramsky O, Teitelbaum D, Arnon R (1977) Experimental allergic neuritis induced by a basic neuritogenic protein (P1L) of human peripheral nerve origin. Eur J Immunol 7:213–217

    Google Scholar 

  • Arnason BGW, Chelmicka-Szorc E (1972) Passive transfer of experimental allergic neuritis in Lewis rats by direct injection of sensitized lymphocytes into sciatic nerve. Acta Neuropathol (Berl) 22:1–5

    Google Scholar 

  • Astrom KE, Waksman BH (1962) The passive transfer of experimental allergic encephalomyelitis and neuritis with living lymphoid cells. J Pathol Bacteriol 83:89–106

    Google Scholar 

  • Feasby TE, Hahn AF, Gilbert JJ (1982) Passive transfer studies in Guillain-Barre polyneuropathy. Neurology 32:1159–1167

    Google Scholar 

  • Hahn AFG, Gilbert J, Feasby TE (1980) Passive transfer of demyelination by experimental allergic neuritis serum. Acta Neuropathol (Berl) 49:169–176

    Google Scholar 

  • Holda JH, Welch AM, Swanborg RH (1980) Autoimmune effector cells. I. Transfer of experimental allergic encephalomyelitis with lymphoid cells cultured with antigen. Eur J Immunol 10:657–659

    Google Scholar 

  • Hughes RAC, Kadlubowski M, Gray I, Leibowitz A (1981) Immune responses in experimental allergic neuritis. J Neurol Neurosurg Psychiatry 44:565–569

    Google Scholar 

  • Kadlubowski M, Hughes RAC, Gregson NA (1980) Experimental allergic neuritis in the Lewis rat: Characterization of the activity of peripheral myelin and its major basic protein P2. Brain Res 184:439–454

    Google Scholar 

  • Norton WT, Poduslo SE (1973) Myelination in rat brain: Changes in myelin composition during brain maturation. J Neurochem 21:759–773

    Google Scholar 

  • Ortiz-Ortiz L, Weigle WO (1976) Cellular events in the induction of experimental allergic encephalomyelitis in rats. J Exp Med 144:604–616

    Google Scholar 

  • Panitch HS, McFarlin DE (1977) Experimental allergic encephalomyelitis: Enhancement of cell-mediated transfer by Concanavalin A. J Immunol 119:1134–1137

    Google Scholar 

  • Paterson PY (1960) Transfer of allergic encephalomyelitis in rats by means of lymph node cells. J Exp Med 3:119–135

    Google Scholar 

  • Raine C, Traugott U, Farooq M, Bornstein M, Norton W (1981a) Augmentation of immune-mediated demyelination by lipid haptens. Lab Invest 45:174–182

    Google Scholar 

  • Raine C, Johnson A, Marcus DM, Suzuki A, Bornstein MB (1981b) Demyelination in vitro. Absorption studies demonstrate that galactocerebroside is a major target. J Neurol Sci 52:117–131

    Google Scholar 

  • Saida K, Saida T, Brown MJ, Silberberg DH, Asbury AK (1978) Antiserum-mediated demyelination in vivo. A sequential study using intraneural injection of experimental allergic neuritis serum. Lab Invest 39:449–462

    Google Scholar 

  • Saida T, Saida K, Dorfman SH, Silverberg G, Summer A, Manning M, Lisak R, Brown M (1979) Experimental allergic neuritis induced by sensitization with galactocerebroside. Science 204: 1103–1106

    Google Scholar 

  • Spencer PS, Weinberg HJ, Raine CS, Prineas JW (1975) The perineural window — a new modal of focal demyelination and remyelination. Brain Res 96:323–329

    Google Scholar 

  • Waksman B, Adams RD (1956) A comparative study of experimental allergic neuritis in the rabbit, guinea-pig and mouse. J Neuropathol Exp Neurol 15:293–332

    Google Scholar 

Download references

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Authors and Affiliations

  1. Dept. of Pathology (Neuropathology), Victoria Hospital, N6A 4G5, London, Ontario, Canada

    J. J. Gilbert, T. E. Feasby & A. F. G. Hahn

  2. Dept. of Clinical Neurological Sciences, University Hospital, London, Ontario, Canada

    J. J. Gilbert, T. E. Feasby & A. F. G. Hahn

  3. University of Western Ontario, London, Ontario, Canada

    J. J. Gilbert, T. E. Feasby & A. F. G. Hahn

Authors
  1. J. J. Gilbert
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  2. T. E. Feasby
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  3. A. F. G. Hahn
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Supported by the Muscular Dystrophy Association of Canada and the Atkinson Charitable Foundation

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Gilbert, J.J., Feasby, T.E. & Hahn, A.F.G. Intraneural injection of lymphocytes in experimental allergic neuritis. Acta Neuropathol 61, 61–64 (1983). https://doi.org/10.1007/BF00688387

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  • DOI: https://doi.org/10.1007/BF00688387

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