Summary
The expression of glial fibrillary acidic protein, fibronectin (FN), factor VIII-related antigen (FVIII/RAG), and of three monohistiocytic markers, lysozyme, α-1-antitrypsin and α-1-antichymotrypsin was examined in five gliosarcomas (GS) by peroxidase-antiperoxidase immunostaining of formalinfixed and paraffin-embedded specimens, and compared with vascular changes in 16 glioblastomas (GB).
In contrast to GB, endothelial proliferations of GS were sheathed by sarcomatous tissue (perivascular sarcoma), which was contiguous with fibrosarcomatous areas. Cells with conspicuous intracytoplasmic FN content (FN+ cells) were seen in the vascular stroma of GB and dominated in the sarcomatous parts of GS. Most FN+ cells of GS were of varying size and shape and clearly neoplastic. Monohistiocytic markers were demonstrable in small infiltrating mononuclear cells as well as in many sarcomatous cells including FN+ cells. FVIII/RAG was restricted to lumen-lining endothelium and was not found in sarcomatous cells.
These results suggest that a major part of sarcoma in GS is less likely to develop from proliferated endothelial cells than from histiocytic cells in the perivascular spaces of GB. By FN mediation, histiocytic cells might also guide and promote sarcomatous proliferations of other mesenchymal cells, leading to fibrosarcomatous development. Prominent monstrous giant cells of one GS seemed to be degenerating glioma cells.
Similar content being viewed by others
References
Alitalo K, Hovi T, Vaheri A (1980) Fibronectin is produced by human macrophages. J Exp Med 151:602–613
Böhling T, Paetau A, Ekblom P, Haltia M (1983) Distribution of endothelial and basement membrane markers in angiogenic tumors of the nervous system. Acta Neuropathol (Berl) 62:67–72
Brem S, Cotran R, Folkman J (1972) Tumor angiogenesis: a quantitative method for histologic grading. J Natl Cancer Inst 48:347–356
D'Ardenne AJ, Kirkpatrick P, Sykes BC (1984) Distribution of laminin, fibronectin, and interstitial collagen type III in soft tissue tumors. J Clin Pathol 37:895–904
Du Boulay CEH (1982) Demonstration of fibronectin in soft tissue tumors using the immunoperoxidase technique. Diagn Histopathol 5:283–289
Du Boulay CEH (1982) Demonstration of alpha-1-antitrypsin and alpha-1-antichymotrypsin in fibrous histiocytomas using the immunoperoxidase technique. Am J Surg Pathol 6:559–564
Eng LF, Rubinstein LJ (1978) Contribution of immunohistochemistry to diagnostic problems of human cerebral tumors. J Histochem Cytochem 26:513–522
Feigin IH, Gross SW (1955) Sarcoma arising in glioblastoma of the brain. Am J Pathol 31:633–653
Feigin I, Allen LB, Lipkin L, Gross SW (1958) The endothelial hyperplasia of the cerebral blood vessels with brain tumors, and its sarcomatous transformation. Cancer 11:264–277
Foulds L (1940) Histological analysis of tumors. A critical review. Am J Cancer 39:1–24
Fu YS, Gabbiani G, Kaye GI, Lattes R (1975) Malignant soft tissue tumors of probable histiocytic origin (malignant fibrous histiocytomas): general considerations and electron microscopic and tissue culture studies. Cancer 35:176–198
Grinnell F, Billingham RE, Burgess L (1981) Distribution of fibronectin during wound healing in vivo. J Invest Dermatol 76:181–189
Hadfield MG, Silverberg SG (1972) Light and electron microscopy of giant-cell glioblastoma. Cancer 30:989–996
Herpers MJHM, Budka H, McCormick D (1984) Production of glial fibrillary acidic protein (GFAP) by neoplastic cells: adaptation to the microenvironment. Acta Neuropathol (Berl) 64:333–338
Holund B, Clemmensen I, Wanning M (1984) Sequential appearance of fibronectin and collagen fibers in experimental arthritis in rabbits. Histochemistry 80:39–44
Hynes RO, Yamada KM (1982) Fibronectin: multifunctional modular glycoproteins. J Cell Biol 95:369–377
Johansson S, Rubin K, Höök M, Ahlgren T, Seljelid R (1979) In vitro biosynthesis of cold insoluble globulin (fibronectin) by mouse peritoneal macrophages. FEBS Lett 105:313–316
Kepes JJ (1986) Neoplastic astrocytes mimicking other tissue elements: diagnostic implications. XVIth International Congress of the International Academy of Pathology, Vienna, August 31–September 5, 1986
Kochi N, Tani E, Morimura T, Itagaki T (1983) Immunohistochemical study of fibronectin in human glioma and meningioma. Acta Neuropathol (Berl) 59:119–126
Kochi N, Budka H, Radaszkiewicz T (1986) Development of stroma in malignant lymphomas of the CNS compared with epidural lymphomas. Acta Neuropathol (Berl) 71: 125–129
Kurkinen M, Vaheri A, Roberts PJ, Stenman S (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 43:47–51
Lalitha VS, Rubinstein LJ (1979) Reactive glioma in intracranial sarcoma: a form of mixed sarcoma and glioma (“sarcoglioma”). Cancer 43:246–257
Lynn JA, Panopio IT, Martin JH, Shaw ML, Race GJ (1968) Ultrastructural evidence for astroglial histogenesis of the monstrocellular astrocytoma (so-called monstrocellular sarcoma of brain). Cancer 22:356–366
McComb RD, Jones TR, Pizzo SV, Bigner DD (1982) Immunohistochemical detection of factor VIII/von Willebrand factor in hyperplastic endothelial cells in glioblastoma multiforme and mixed glioma-sarcoma. J Neuropathol Exp Neurol 41:479–489
Möller P, Achtstätter H, Butzengeiger M, Schüle B (1983) The distribution of fibronectin in lymph nodes infiltrated by Hodgkin's disease. Virchows Arch [A] 400:319–329
Morantz RA, Feigin I, Ransohoff J III (1976) Clinical and pathological study of 24 cases of gliosarcoma. J Neurosurg 45:398–408
Oehmischen M (1982) Functional properties of microglia. In: Smith WT, Cavanagh JB (eds) Recent advances in neuropathology, vol 2. Churchill Livingstone, Edinburgh, pp 83–107
Papadimitriou CS, Stephanaki-Nikou SN, Malamou-Mitsi VD (1983) Comparative immunostaining of T-associated plasma cells and other lymph-node cells in paraffin sections. Virchows Arch [Cell Pathol] 43:31–36
Pena CE, Felter R (1973) Ultrastructure of a composite glioma-sarcoma of the brain. Acta Neuropathol (Berl) 23:90–94
Peters A, Palay S, Webster H (1979) The fine structure of the nervous system. Saunders, Philadelphia, p 254
Radzun HJ (1985) Immunohistochemie des menschlichen mononukleär-phagozytischen Systems. Gustav Fischer, Stuttgart, pp 93–96
Ree HJ, Kadin ME (1985) Lectin distinction of benign from malignant histiocytes. Cancer 56:2046–2050
Reilly JT, Nash JR, Mackie MJ, McVerry BA (1985) Distribution of fibronectin and laminin in normal and pathological lymphoid tissue. J Clin Pathol 38:849–854
Rubinstein LJ (1964) Morphological problems of brain tumors with mixed cell population. Acta Neurochir (Wien) [Suppl] 10:141–165
Rubinstein LJ (1972) Tumors of the central nervous system. Armed Forces Institute of Pathology, Washington, DC, pp 74–78 and 198–199
Russel DS, Rubinstein LJ (1971) Pathology of tumours of the nervous system, 3rd edn. Edward Arnold, London, pp 179–181
Schiffer D, Giordana MT, Soffietti R, Tarenzi L, Bertolotto A (1980) On the nature of the so-called monstrocellular sarcoma of the brain. Neurosurgery 6:391–397
Schiffer D, Giordana MT, Mauro A, Migheli A (1984) GFAP, FVIII/RAG, laminin, and fibronectin in gliosarcomas: an immunohistochemical study. Acta Neuropathol (Berl) 63:108–116
Slowik F, Jellinger K, Gaszo L, Fischer J (1985) Gliosarcomas: histological, immunohistochemical, ultrastructural, and tissue culture studies. Acta Neuropathol (Berl) 67:201–210
Smith DR, Hardman JM, Earle KM (1969) Contiguous gliosarcoma multiforme and fibrosarcoma with extracranial metastasis. Cancer 24:270–276
Stroebe H (1895) Über Entstehung und Bau der Gehirngliome. Beitr Pathol Anat Allg Pathol 18:405–486
Van de Water L III, Schroeder S, Crenshaw EB III, Hynes RO (1981) Phagocytosis of gelatin-latex particles by a murine macrophage line is dependent on fibronectin and heparin. J Cell Biol 90:32–39
Villiger B, Kelley DG, Engleman W, Kuhn C III, McDonald JA (1981) Human alveolar macrophage fibronectin: synthesis, secretion, and ultrastructural localization during gelatin-coated latex particle binding. J Cell Biol 90:711–720
Weiss SW, Enzinger FM (1978) Malignant fibrous histiocytoma: an analysis of 200 cases. Cancer 41:2250–2266
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kochi, N., Budka, H. Contribution of histiocytic cells to sarcomatous development of the gliosarcoma. Acta Neuropathol 73, 124–130 (1987). https://doi.org/10.1007/BF00693777
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00693777