Summary
Dorsal root ganglion cells, obtained from 8–10-week human foetuses, were isolated by enzymatic procedure and grown on poly-l-lysine-coated coverslips. Most of the cultured cells showed the ultrastructural and immunological features of normal peripheral neurons. By immunocytochemistry neurons reacted with IgM antibodies with specificity for myelin-associated glycoprotein (MAG) from patients affected with IgM k gammopathy and peripheral neuropathy. The antigen was located on the plasmalemma of both perikarion and axon. We suggest that anti-MAG antibodies do not recognize neuronal MAG, but rather an epitope shared with different glycoproteins.
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Moretto, G., Monaco, S., Nardelli, E. et al. Binding of monoclonal anti-myelin-associated glycoprotein antibodies to human foetal peripheral neurons in culture. Acta Neuropathol 73, 344–348 (1987). https://doi.org/10.1007/BF00688257
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DOI: https://doi.org/10.1007/BF00688257