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Beitrag zur Differenzierung von Nerven- und Gliazellen mit Hilfe der Silberimprägnation

Elektronenmikroskopische Untersuchung

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Zusammenfassung

Das Gewebe der Großhirnrinde wurde elektronenmikroskopisch nach Silber-imprägnation (Marx u.Mölbert, 1965) und nach Bleihydroxydkontrastierung untersucht.

Im Bereich des Neuropils konnten die Basalmembranen der Capillaren lediglich aus der Lamina densa bestehend beobachtet werden. Die Neurofibrillen der Axone waren stark versilberbar. In den Nervenzellen konnten erstmals Mikrotubuli, die sich von den Neurofibrillen durch ihre verschiedene Kontrastierbarkeit unterscheiden gefunden werden. Ferner konnten “reticular inclusions” im Bereich des endoplasmatischen Reticulums dargestellt werden. Die Unterscheidbarkeit von Nervenzellen einerseits und Astrocyten und Oligodendrocyten andererseits mit Hilfe der Silberimprägnation wird aufgezeigt.

Summary

Brain tissue was investigated with the electron microscope. The tissue was stained with silver or lead hydroxide. The capillaries of the neuropil have base membranes which consist of a layer only, the lamina densa. The neurofibriles of the axons can be stained readily by silver. In the nerve cells microtubules can be traced. They differ from the neurofibrils. Surrounded by endoplasmic reticulum, reticular inclusions can be found. Because of the impregnation with silver one can easily differentiate between nerve cells, astrocytes and oligodendrocytes.

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Authors and Affiliations

  1. Ludwig Aschoff-Haus, Pathologisches Institut der Universität Freiburg im Breisgau, Freiburg im Breisgau, Deutschland

    U. -P. Ketelsen, R. Marx & E. Mölbert

  2. Dept. of Physics Physical Science Building, Kansas State University, 66502, Manhatten, Kansas, USA

    E. Mölbert

Authors
  1. U. -P. Ketelsen
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  2. R. Marx
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  3. E. Mölbert
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Ketelsen, U.P., Marx, R. & Mölbert, E. Beitrag zur Differenzierung von Nerven- und Gliazellen mit Hilfe der Silberimprägnation. Acta Neuropathol 9, 185–197 (1967). https://doi.org/10.1007/BF00691443

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