Skip to main content
SpringerLink
Log in

Verschiedene Formen des endoplasmatischen Reticulums in entdifferenzierten Keratinocyten

Beobachtungen am Plattenepithel-Carcinom

Different forms of the endoplasmic reticulum in neoplastic keratinocytes

Investigations on the Squamous Cell Carcinoma

  • Published:
Archiv für dermatologische Forschung Aims and scope Submit manuscript

Summary

Electron microscopic examination of squamous cell carcinoma of human epidermis showed different formations of the endoplasmic reticulum (ER) in neoplastic keratinocytes, which are unusual for normal epidermal cells. They can be characterized as follows:

  1. 1.

    Some cells show no ER and only few ribosomes.

  2. 2.

    Other cells demonstrate increased proliferation of the rough surfaced ER and the free polyribosomes.

  3. 3.

    One part of the cells shows a strong increase of free ribosomes, while scanty granulated tubes of rough surfaced ER are fragmented and diffusely distributed.

  4. 4.

    A hydropic dilatation of the ER is a predominant feature of some cells.

  5. 5.

    Smooth surfaced endoplasmic reticulum appears frequently, mostly arranged to systems of lamellae like “whorls” or “finger prints”. Alterationes of membranes can lead to cytolysis and to the formation of cytolysosomes.

  6. 6.

    Lipid droplets are recognized, more often in connection to the ER.

The morphological and also the functional abnormalities are not specific. The example of the neoplastic keratinocytes shows that malignant growth generally does not coincide with decreased cytoplasmic differentiation.

Zusammenfassung

Beim Plattenepithel-Carcinom menschlicher Epidermis zeigen entdifferenzierte Keratinocyten verschiedene Formationen des endoplasmatischen Reticulums (ER), die für normale Epidermiszellen ungewöhnlich sind. Sie lassen sich folgendermaßen charakterisieren:

  1. 1.

    Einige Zellen zeigen keinerlei ER und nur wenige Ribosomen.

  2. 2.

    Andere lassen eine starke Proliferation des rauhen ER und der freien Polyribosomen erkennen.

  3. 3.

    Ein Teil der Zellen zeigt eine starke Vermehrung freier Ribosomen, während spärlich granulierte ER-Schläuche eine Fragmentation und diffuse Verteilung aufweisen.

  4. 4.

    Eine hydropische Dilatation des ER beherrscht das Zellbild.

  5. 5.

    Glattes ER tritt gehäuft auf, oft als „whorls“ oder „finger prints“ zu Lamellensystemen geordnet. Membranschäden können zur Cytolyse und zur Bildung von Cytolysosomen führen.

  6. 6.

    In Beziehung zum ER lassen sich vermehrt Lipidtropfen erkennen.

Die morphologischen und damit auch funktionellen Abweichungen von der Norm sind unspezifischer Natur. Sie zeigen am Beispiel der malignen Keratinocyten, daß malignes Wachstum nicht generell mit einer Abnahme der Cytoplasmadifferenzierung einhergeht.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Abercrombie, M., Ambrose, E. J.: The surface properties of cancer cells: a review. Cancer Res.22, 525–548 (1962).

    Google Scholar 

  • Ardenne, M. v., Rieger, F.: Zu den Wegen der Krebstherapie und möglichen Ursachen der Krebsentstehung sowie ihren wechselseitigen Beziehungen. Z. Naturforsch.23 b, 357–368 (1968).

    Google Scholar 

  • Bernhard, W.: Elektronenmikroskopischer Beitrag zum Studium der Kanzerisierung und der malignen Zustände der Zelle. Verh. dtsch. Ges. Path.45, 8–39 (1961).

    Google Scholar 

  • —: Cancer Cell. In: Handbook of molecular cytology, pp. 687–715. Edit. by A. Lima-De-Faria. Amsterdam-London: North-Holland-Publ. Comp. 1969.

    Google Scholar 

  • Borland, R., Webber, A. J.: An electron microscope study of squamous cell carcinoma in Merino sheep associated with keratin-filled cysts of the skin. Cancer Res.26, 172–182 (1966).

    Google Scholar 

  • Bhowmick, D. K., Mitra, A.: Ultrastructural morphology of squamous carcinoma of human exocervix. Indian J. med. Res.56, 282–287 (1968).

    Google Scholar 

  • Brody, I.: The epidermis. In: Handbuch Hautkrankheiten, Ergänzungswerk. I/1. Berlin-Heidelberg-New York: Springer 1968.

    Google Scholar 

  • David, H.: Elektronenmikroskopische Organpathologie. Berlin: VEB Volk und Gesundheit 1967.

    Google Scholar 

  • —: Zellschädigung und Dysfunktion. Protoplasmatologia X, 1. Wien-New York: Springer 1970.

    Google Scholar 

  • Emmelot, P., Benedetti, E. L.: Changes in the fine structure of rat liver cells brought by dimethylnitrosamine. J. biophys. biochem. Cytol.7, 393–396 (1960).

    Google Scholar 

  • —: Some observations on the effect of liver carcinogens in fine structure and function of the endoplasmic reticulum of rat liver cells. Symp. “Protein Synthesis”, pp. 99–123. London: Academic Press Ltd. 1960.

    Google Scholar 

  • Fasske, E., Morgenroth, K., Themann, H., Verhagen, A.: Elektronenmikroskopische Untersuchungen menschlicher Collumcarcinome und ihres Stromas. Arch. Gynäk.192, 571–590 (1960).

    Google Scholar 

  • —, Themann, H.: Die elektronenmikroskopische Struktur menschlicher Carcinome. Beitr. path. Anat.122, 313–344 (1960).

    Google Scholar 

  • Fawcett, D. W.: The membranes of cytoplasm. Lab. Invest.10, 1162–1188 (1961).

    Google Scholar 

  • —: The cell, an atlas of fine structure. Philadelphia-London: W. B. Saunders Comp. 1966.

    Google Scholar 

  • Glatthaar, E., Vogel, A.: Zur Ultrastruktur des Plattenepithel-Karzinoms der Portio. Gynaecologia (Basel)151, 212–226 (1961).

    Google Scholar 

  • Goldblatt, P. J.: The endoplasmic reticulum. In: Handbook of cytology, pp. 1102 to 1129. Edit. by A. Lima-De-Faria. Amsterdam-London: North-Holland-Publ. Comp. 1969.

    Google Scholar 

  • Grundmann, E.: Die Zytogenese des Krebses. Dtsch. med. Wschr.86, 1077–1084 (1961).

    Google Scholar 

  • Haam, E. v.: Die experimentelle Erzeugung des Portio-Karzinoms und seine Vorstufen in Versuchstieren. Verh. dtsch. Ges. Path.48, 57–74 (1964).

    Google Scholar 

  • Hadjolov, A. A.: A biochemical and ultrastructural study on the ergastoplasmic lipoprotein structures in experimental rat liver carcinogenesis. Acta anat. (Basel)61, 622 (1965).

    Google Scholar 

  • Herdson, P. B., Garvin, P. J., Jennings, R. B.: Fine structural changes in rat liver induced by phenobarbital. Lab. Invest.13, 1032–1037 (1964).

    Google Scholar 

  • Hirone, T., Eryu, Y.: Fine structure of squamous cell carcinoma. Skin Res. (Osaka)12, 352–368 (1970).

    Google Scholar 

  • Hutterer, F., Schaffner, F., Klion, F. M., Popper, H.: Hypertrophic, hypoactive smooth endoplasmic reticulum: A sensitive indicator of hepatotoxicity exemplified by dieldrin. Science161, 1017–1019 (1968).

    Google Scholar 

  • Haguenau, F.: The ergastoplasm: its history, ultrastructure and biochemistry. Int. Rev. Cytol.7, 425–483 (1958).

    Google Scholar 

  • Klehr, H. U., Klingmüller, G.: Struktur und Funktion der granulären Kernkörperchen. Beobachtungen am Plattenepithelcarcinom. Arch. klin. exp. Derm.238, 44–52 (1970).

    Google Scholar 

  • Klingmüller, G., Klehr, H. U., Ishibashi, Y.: Desmosomen im Cytoplasma entdifferenzierter Keratinocyten des Plattenepithelcarcinoms. Arch. klin. exp. Derm.238, 356–365 (1970).

    Google Scholar 

  • Lever, W. F., Hashimoto, K.: Electron microscopic and histochemical findings in basal cell epithelioma, squamous cell carcinoma and some appendage tumors. XIII. Congress Internationalis Dermatologiae, Berlin. Main Theme I, 3–8. Berlin-Heidelberg-New York: Springer 1968.

    Google Scholar 

  • Mölbert, E.: Die Orthologie und Pathologie der Zelle im elektronenmikroskopischen Bild. In: Handbuch der allgemeinen Pathologie, Bd. II, T. 5/I, S. 238–465. Berlin-Heidelberg-New York: Springer 1968.

    Google Scholar 

  • Nakai, T., Shubik, P., Feldmann, R.: An electron microscopic study of skin carcinogenesis in the mouse with special reference to the intramitochondrial body. Exp. Cell Res.27, 608–611 (1962).

    Google Scholar 

  • Ormea, F., Bossi, G., Garcovich, A.: Contributo allo studio dell'ultrastruttura delle neoplasie epiteliali cutanee. Minerva derm.43, 683–724 (1968).

    Google Scholar 

  • Orrenius, S. J., Ericsson, L., Ernster, L. E.: Phenobarbital-induced synthesis of microsomal drug-metabolizing enzyme system and its relationship to the proliferation of endoplasmic membranes. J. Cell Biol.25, 627–639 (1965).

    Google Scholar 

  • Palade, G. E.: The endoplasmic reticulum. J. biophys. biochem. Cytol.2, Suppl. 85 (1956).

    Google Scholar 

  • —, Siekevitz, P.: Correlated structural and chemical analysis of microsomes. Fed. Proc.14, 262 (1955).

    Google Scholar 

  • Pillai, P. A., Gautier, A.: Apriliminary note on the electron microscopy of induced epidermal hyperplasia in the newt. Oncologia (Basel)13, 303–310 (1960).

    Google Scholar 

  • Pitot, H. C.: Endoplasmic reticulum and phenotypic variability in normal and neoplastic liver. Arch. Path.87, 212–222 (1969).

    Google Scholar 

  • —, Cho, Y. S.: Control mechanisms in the normal and neoplastic cell. Progr. exp. Tumor Res. (Basel)7, 158–223 (1965).

    Google Scholar 

  • Porter, K. R.: The ground substance: observations from electron microscopy. In: The cell (J. Brachet and A. E. Mirsky, eds.), Vol. II, pp. 621–672. New York: Academic Press 1961.

    Google Scholar 

  • —, Bruni, C.: An electron microscope study of the early effects of 3-Me-DAB on rat liver cells. Cancer Res.19, 997–1009 (1959).

    Google Scholar 

  • Schlunk, F. F., Lombardi, B.: Liver liposomes. I. Isolation and chemical characterization. Lab. Invest.17, 30–38 (1967).

    Google Scholar 

  • Setälä, K., Merenmies, L., Stjernvall, L., Nyholm, N., Aho, Y.: Mechanism of experimental tumorgenesis. V. Ultrastructural alterations in mouse epidermis caused by Span 60 and Tween 60-type agents. J. nat. Cancer Inst.24, 355–385 (1960a).

    Google Scholar 

  • —, Niskanen, E. E., Merenmies, L., Nyholm, M., Stjernvall, L.: Mechanism of experimental tumorgenesis. VI. Ultrastructural alterations in mouse epidermis caused by locally applied carcinogen and dipole-type tumor promotor. J. nat. Cancer Inst.25, 1155–1189 (1960b).

    Google Scholar 

  • Stahl, J., Bielka, H.: Lokalisation und Organisation von Ribosomen in Leber- und Hepatomgewebe. Acta biol. med. germ.19, 249–258 (1967).

    Google Scholar 

  • Stenger, R. J.: Concentric lamellar formations in hepatic parenchymal cells of carbon tetrachloride-treated rats. J. Ultrastruct. Res.14, 240–253 (1966).

    Google Scholar 

  • Sugar, J.: Electron-microscopic study of acid phosphatase and cell-organelles during human and experimental skin carcinogenesis. Acta morph. Acad. Sci. hung.15, 93–108 (1967).

    Google Scholar 

  • —: A electron study of early microscopic invasive growth in human skin tumors and laryngeal carcinoma. Europ. J. Cancer4, 33–38 (1968).

    Google Scholar 

  • Svoboda, D. J.: Fine structure of hepatomas induced in rats with p-Dimethylaminoazobenzene. J. nat. Cancer Inst.33, 315–339 (1964).

    Google Scholar 

  • Tarin, D.: Sequential electron microscopical study of experimental mouse skin carcinogenesis. Int. J. Cancer2, 195–211 (1967).

    Google Scholar 

  • Wallach, D. F. H.: Cellular membrane alterations in neoplasia: a review and a unifying hypothesis. Curr. Top. Microbiol. Immunol.47, 152–176 (1969)

    Google Scholar 

  • —: Generalized membrane defects in cancer. New Engl. J. Med.280, 761–767 (1969).

    Google Scholar 

  • Webb, T. E., Blobel, G., Potter, V. R., Morris, H. P.: Polyribosomes in rat tissues. II. The polysome distribution in the minimal deviation hepatomas. Cancer Res.25, 1219–1224 (1965).

    Google Scholar 

  • Wessel, W.: Endometriale Adenocarcinome verschiedener Differenzierungsgrade und ihr Stroma im elektronenmikroskopischen Bild. Z. Zellforsch.66, 421–437 (1965).

    Google Scholar 

  • Zelickson, A. S.: Ultrastructure of normal and abnormal skin. Philadelphia: Lea and Febiger 1967.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Universitäts-Hautklinik, Bonn, BRD

    H. U. Klehr & G. Klingmüller

Authors
  1. H. U. Klehr
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Klingmüller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klehr, H.U., Klingmüller, G. Verschiedene Formen des endoplasmatischen Reticulums in entdifferenzierten Keratinocyten. Arch. Derm. Forsch. 242, 111–126 (1972). https://doi.org/10.1007/BF00595560

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00595560

Search

Navigation