Summary
Both auricles of 21 domestic rabbits were painted with dimethylbenz-anthracene (DMBA). Eleven animals of this group were additionally fed aromatic retinoid (AR) by an esophageal tube. Two control animals were not treated at all.
Eight or 9 weeks after the beginning of the study six of the seven remaining animals, which had only been painted with DMBA, developed a total of 25 keratoacanthoma-like tumors (KA). On the other hand, none of the seven animals left, which were painted with DMBA and fed AR showed any tumor by this time.
The systemic effect of AR was studied in biopsies from the snout and the back. The epidermis of the snout showed ‘mucous mataplasia’ by histochemical and electron-microscopic criteria, whereas the epidermis of the back was not significantly altered. The production of intra- and extracellular lamellated material indicated an additional effect of AR on epidermal lipid metabolism. The effect of AR in the prevention of DMBA-induced tumors was characterized by ‘mucoid cytolysis’ and karyolysis.
Similar content being viewed by others
References
Lotan R (1980) Effects of vitamin A and its analogs (retinoids) on normal and neoplastic cells. Biochim Biophys Acta 605:33–91
Orfanos CE (1980) Oral retinoids — present status. Br J Dermatol 103:473–481
Elias PM, Williams ML (1981) Retinoids, cancer, and the skin. Arch Dermatol 117:160–180
Mahrle G, Berger H (1981) Antitumoreffekt der Retinoide. Z Hautkr (in press)
Braun-Falco O, Christophers E (1969) Psoriasiforme Epidermisreaktion der Meerschweinchenhaut durch örtliche Vitamin A-Säure-Applikation. Arch Klin Exp Dermatol 234:70–86
Tsambaos D, Mahrle G, Orfanos CE (1980) Epidermal changes induced by oral excess of aromatic retinoid in guinea pigs. Arch Dermatol Res 267:141–152
Levij IS, Polliack A (1968) Potentiating effect of vitamin A on 9–10 dimethyl 1–2 benzanthrancene carcinogenesis in the hamster cheek pouch. Cancer 22:300–306
Smith DM, Rogers AE, Herndon BJ, Newberne PM (1975) Vitamin A (retinyl acetate) and benzo(a)pyrene-induced respiratory tract carcinogenesis in hamsters fed a commercial diet. Cancer Res 35:11–16
Epstein JH (1977) Chemicals and photocarcinogenesis. Aust J Dermatol 18:57–61
Forbes PD, Urbach F, Davies RE (1979) Enhancement of experimental photocarcinogenesis by topical retinoic acid. Cancer Lett 7:85–90
Prutkin L (1975) Mucous metaplasia and gap junctions in the vitamin A acid-treated skin tumor, keratoacanthoma. Cancer Res 35:364–369
Mahrle G, Meyer-Hamme S, Ippen H (1981) Systemic treatment of keratinizing disorders of the skin and the mucous membrane with an aromatic retinoid (Ro 10-9359). Comparative study on 113 patients. Arch Dermatol (in press)
Mayer H, Bollag W, Hänni R, Rüegg R (1978) Retinoids a new class of compounds with prophylactic and therapeutic activities in oncology and dermatology. Experientia 34:1105–1246
Schultz-Ehrenburg U, Orfanos CE (1981) Light and electron microscopic changes of human epidermis under oral retinoid treatment. In: Orfanos CE, Braun-Falco O, Farber EM, Grupper Ch, Polano MK, Schuppli R (eds) Retinoids. Advances in basic research and therapy. Springer, Berlin Heidelberg New York, pp 5–11
Harms V, Olmos L, Hunziker N, Laugier P (1976) Das experimentelle Keratoakanthom am Kaninchenohr (KA). Arch Dermatol Res 257:131–142
Levinson SS, Wolf G (1972) The effect of vitamin A acid on glycoprotein synthesis in skin tumors (keratoacanthomas). Cancer Res 32:2248–2252
Baer RL, Kopf AW (1962) Keratoacanthoma. In: Baer RL, Kopf AW (eds) The year book of dermatology. Year Book Medical Publishers Chicago, pp 7–41
Matter A, Bollag W (1977) A fine structural study on the therapeutic effect of an an aromatic retioid on chemically induced skin papillomas of the mouse. Eur J Cancer 13:831–838
Yuspa SH, Hennings H, Saffiotti U (1976) Cutaneous chemical carcinogenesis: Past, present, and future. J Invest Dermatol 67:199–208
Iversen OH (1974) Dynamic aspects of epidermal skin carcinogenesis. In: Flarer F, Servi F, Cotton DWK, (eds) Dermatology. Proceedings of the XIV International Congress, Padua-Venice, May 22–27, 1972. Excerpta Medica, Amsterdam, pp 552–554
Mahrle GO, Wilkinson DI, Farber EM (1981) Effect of retinoids on keratinocytes in vitro. In: Orfanos CE, Braun-Falco O, Farber EM, Grupper Ch, Polano MK, Schuppli R (eds) Retinoids. Advances in basic research and therapy. Springer, Berlin Heidelberg New York, pp 145–152
Mahrle GO, Gray B (1981) The effect of vitamin A acid on homeostasis and differentiation of epidermal cells cultured on collagen. Dermatologia (submitted)
Author information
Authors and Affiliations
Additional information
Supported by the Deutsche Forschungsgemeinschaft (Ma 674/3-2)
Rights and permissions
About this article
Cite this article
Mahrle, G., Berger, H. DMBA-induced tumors and their prevention by aromatic retinoid (Ro 10-9359). Arch Dermatol Res 272, 37–47 (1981). https://doi.org/10.1007/BF00510391
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00510391