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Comparison of differentiation patterns in 12-O-tetradecanoylphorbol-13-acetate-treated epidermis and fetal epidermis

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Summary

12-O-Tetradecanoylphorbol-13-acetate (TPA)-treated adult epidermis as well as untreated fetal and adult epidermis were investigated to elucidate the effect of TPA in terms of cell differentiation using techniques of ultrastructural stereology. Twenty-four hours after a single application of TPA, the treated epidermis was characterized by involutional changes, i. e., increased volume density of intercellular spaces and of mitochondria, vacuoles, and cytoplasmic ground substance in the basal layer. However, 48 h after application, the TPA-treated epidermis was very similar to fetal epidermis, i. e., high volume density of nuclei ribosomes, rough endoplasmic reticulum, membrane-coating granules, and keratohyalin granules, and low volume density of bundled filaments in the upper layers. These stereological data indicate that the changes observed 48 h after TPA treatment were related not only to increased cell proliferation but also to inhibition of cell differentiation expressed as a reversion of the adult differentiation patterns and the acquisition of fetal characteristics in all epidermal layers.

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Abbreviations

TPA:

12-O-tetradecanoylphorbol-13-acetate

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Author notes

  1. M. Chiba

    Present address: Department of Dermatology, Sapporo Medical College, Minami 1, Nishi 16, Sapporo, Japan

Authors and Affiliations

  1. Science Park-Research Division, University of Texas System Cancer Center, P. O. Box 389, 78957, Smithville, TX, USA

    M. Chiba, C. M. Taylor & A. J. P. Klein-Szanto

Authors
  1. M. Chiba
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  2. C. M. Taylor
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  3. A. J. P. Klein-Szanto
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Additional information

This research was supported by PHS grant CA 34690 awarded by the National Cancer Institute, NIH DHHS

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Chiba, M., Taylor, C.M. & Klein-Szanto, A.J.P. Comparison of differentiation patterns in 12-O-tetradecanoylphorbol-13-acetate-treated epidermis and fetal epidermis. Arch Dermatol Res 277, 457–465 (1985). https://doi.org/10.1007/BF00510063

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  • DOI: https://doi.org/10.1007/BF00510063

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