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Kallikrein excretion in parotid saliva in rats with various forms of arterial hypertension

Kallikrein-Ausscheidung im Parotisspeichel von Ratten mit verschiedenen Hochdruckformen

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Zusammenfassung

Es wird eine Methode beschrieben für die fortlaufende, quantitative Untersuchung des Parotisspeichels von Ratten unter Berücksichtigung der Flußrate. Bei verschiedenen Formen des arteriellen Hochdruckes (genuiner, renovaskulärer, DOCTMA-Hochdruck) konnte so die Konzentration des Kallikreins im Parotisspeichel bestimmt werden. Die Kallikrein-Sekretion wurde gemessen an ihrer esterolytischen Aktivität.

Die Exkretion des Kallikreins lag bei normotensiven Kontrolltieren zwischen 2,5 und 4,0 mU/min während einer durch Pilokarpin ausgelösten Stimulation der Drüsenfunktion. Bei allen Formen des arteriellen Hochdrucks war die Sekretion von Kallikrein um das zwei- bis vierfache erhöht. Dieser Anstieg hatte keine Beziehung zur Aktivität des Renin-Angiotensin-Systems.

Summary

A technique for continuous and quantitative collection of parotid saliva — including salivary flow rate determination — for in vivo experiments in rats is described. Excretion of kallikrein-like activity in parotid saliva of rats with various forms of arterial hypertension (genuine, renovascular and DOCTMA-salt hypertension) was studied. Kallikrein excretion was measured by its esterolytic activity.

The levels of kallikrein-like activity in parotid saliva of normotensive control rats ranged between 2.5–4.0 mU/min during salivary flow stimulation with pilocarpine. In all forms of experimental hypertension salivary excretion of kallikrein-like activity was increased 2–4 fold. This increase was not related to the activity of the renin-angiotensin system.

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Authors and Affiliations

  1. Abteilung Neprologie, Medizinische Universitätsklinik Würzburg, Josef-Schneider-Straße 2, D-8700, Würzburg, Federal Republic of Germany

    G. Schmid, G. P. Dragoun & A. Heidland

  2. Klinik und Poliklinik für Hals-Nasen-Ohrenkranke der Universität Erlangen-Nürnberg, Waldstraße 1, D-8520, Erlangen, Federal Republic of Germany

    M. E. Wigand

Authors
  1. G. Schmid
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  2. G. P. Dragoun
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  3. M. E. Wigand
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  4. A. Heidland
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Dedicated to Professor Dr. H. A. Kühn on occasion of his 65th birthday

Supported by the Deutsche Forschungsgemeinschaft, SFB 92, Biomund

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Schmid, G., Dragoun, G.P., Wigand, M.E. et al. Kallikrein excretion in parotid saliva in rats with various forms of arterial hypertension. Arch Otorhinolaryngol 225, 185–190 (1979). https://doi.org/10.1007/BF00455253

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  • DOI: https://doi.org/10.1007/BF00455253

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