Summary
A method for middle ear epithelial (CMEE) cell culture with active mucus secretory function has been successfully developed, using the chinchilla as an animal model. CMEE cells were dissociated by protease digestion from the middle ear mucosa. The CMEE cells grown in primary culture incorporated [3H] glucosamine into a glycoconjugate after its release into medium. This substance was characterized biochemically as mucin, although the production of mucin by the cells required growth on a substratum of collagen gel. These cultures provide an excellent model for studying factors that regulate synthesis and secretion of glycoproteins in CMEE cells.
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References
Chopra DP, Sullivan J, Wille JJ, Siddiqui KM (1987) Propagation of differentiating normal human tracheobronchial epithelial cells in serum-free medium. J Cell Physiol 130:173–181
Coles SJ, Bhaskar KR, O'Sullivan DD, Neill KH, Reid LM (1984) Airway mucus: composition and regulation of its secretion by neuropeptides in vitro. In: Nugent J, O'Connor M (eds) Mucus and mucosa. Ciba Found Symp 109. Pitman; London, pp 40–60
Harada T, Kim Y, Juhn SK (1989) Identification and culture of mucosal epithelial cells from chinchilla middle ear (abstract). 12th Midwinter Meeting of Association for Research in Otolaryngology, St. Petersburg Beach, Florida, pp 211–212
Kim KC (1985) Possible requirement of collagen gel substratum for production of mucin-like glycoproteins by primary rabbit tracheal epithelial cells in culture. In Vitro Cell Dev Biol 21:617–621
Laurent C, Tengblad A, Engstrom-Laurent A (1986) Hyaluronic acid in secretory otitis media. A biochemical study of mucoid middle-ear effusions and serum. J Larnygol Otol 100: 135–140
Nakamura A, DeMaria TF, Lim DJ, Van Blitteswijk C (1990) Serial culture and characterization of the chinchilla middle ear epithelium (abstract). 13th Midwinter Meeting of Association for Research in Otolaryngology, St. Petersburg Beach, Florida, p 39
Nakazawa K, Newsome DA, Nilsson B, Hascall VC, Hassell JR (1983) Purification of keratan sulfate proteoglycan from monkey cornea. J Biol Chem 258:6051–6055
Rearick JI, Deas M, Jetten AM (1987) Synthesis of mucous glycoproteins by rabbit tracheal cells in vitro. Modulation by substratum; retinoids and cyclic AMP. Biochem J 242:19–25
Takasaki S, Kobata A (1978) Microdetermination of sugar compositon by radioisotope labeling. Methods Enzymol 50: 50–54
Takeno S, Hirakawa K, Kasuga S, Harada Y (1989) Cell culture of middle ear epithelium of the guinea pig. Ear Res Jpn 20:143–144
Van Blitterswijk CA, Ponce M, Van Muijen GNP, Wijsman MC, Koerten HK, Grote JJ (1986) Culture and characterization of rat middle-ear epithelium. Acta Otolaryngol (Stockh) 101:453–466
Wu R (1986) In vitro differentiation of airway epithelial cells. In: Schiff LJ (ed) In vitro models of respiratory epithelium. CRC Press, Boca Raton, pp 1–26
Wu R, Yanakaskas J, Cheng E, Knowles MR, Boucher R (1985) Growth and differentiation of human nasal epithelial cells in culture: serum-free hormone-supplemented medium and proteoglycan synthesis. Am Rev Respir Dis 132:311–320
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Haradal, T., Kim, Y., Juhn, S.K. et al. Biochemical characterization of mucous glycoproteins secreted by in vitro chinchilla middle ear epithelial cells. Eur Arch Otorhinolaryngol 250, 182–185 (1993). https://doi.org/10.1007/BF00171708
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DOI: https://doi.org/10.1007/BF00171708