Summary
The concentrations of methadone in human hair were measured. The washed hair was cut in 1-mm pieces approximately, then incubated overnight at 45°C with 0.1 m HCl. The extracts were alcalized by 1 m NaOH and diluted by phosphate buffer, pH 7.4. The methadone concentrations were determined by radioimmunoassay. The method is simple, rapid, and practicable for routine determination.
Zusammenfassung
Die Konzentration von Methadon im menschlichen Haar wurde bestimmt. Die gewaschenen Haarproben wurden in ca. 1 mm lange Stücke geschnitten und mit 0.1 m HCl über Nacht bei 45°C inkubiert. Die Extrakte wurden mit 1 m NaOH alkalisiert und mit Phosphat-Puffer pH 7.4 verdünnt. Die Methadonkonzentrationen wurden mittels Radioimmunoassay gemessen. Diese Methode stellt eine einfache und schnelle Routinebestimmung dar.
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Balabanova, S., Wolf, H.U. Determination of methadone in human hair by radioimmunoassay. Z Rechtsmed 102, 1–4 (1989). https://doi.org/10.1007/BF00201255
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DOI: https://doi.org/10.1007/BF00201255