Summary
A rapid protocol has been established for sexing forensic samples by the Polymerase Chain Reaction method. Three sets of primer were used, two specific for Y chromosome repetitive sequences and one specific for X chromosome repetitive sequences. Detailed procedures of experiments, the controls and the applications to testing bloodstains and a vaginal swab are presented. The sensitivity of the test and problems due to contamination are discussed.
Zusammenfassung
Wir haben ein Schnellprotokoll zur Geschlechtsbestimmung forensischer Proben mit Hilfe der „Polymerase Chain Reaction” etabliert. Hierzu werden 3 Primer-Paare verwendet; 2 hiervon sind spezifisch für repetitive Sequenzen auf dem Y-Chromosom, ein Paar ist spezifisch für repetitive Sequenzen auf dem X-Chromosom. Die Methode wird detailliert beschrieben, ebenfalls die durchgeführten Kontrollen. Die Anwendungsmöglichkeiten an Blutflecken und Vaginalabstrichen werden vorgestellt. Erwähnung findet auch die Empfindlichkeit des Tests und die Problematik, welche durch Verunreinigungen der Spur entsteht.
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Pascal, O., Aubert, D., Gilbert, E. et al. Sexing of forensic samples using PCR. Int J Leg Med 104, 205–207 (1991). https://doi.org/10.1007/BF01369808
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DOI: https://doi.org/10.1007/BF01369808