Abstract
In order to develop a stain with increased sensitivity and selectivity for cadmium (Cd), we synthesized and characterized 2-(8-quinolylazo)-4,5-diphenylimidazole (QAI). This chelating agent was more than twice as sensitive for Cd than the best conventional staining agents, including benzothiazolylazo-beta-naphthol. Differentiation between Cd and zinc (Zn) was achieved by immersing tissue sections in TRIS(2-aminoethyl)amine before they were stained with QAI. This pretreatment made it possible to selectively stain for Cd by blocking Zn.
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References
Friberg L (1950) Health hazards in the manufacture of alkaline accumulators with special reference to chronic cadmium poisoning. Acta Med Scand 138 [Suppl 240]:35–40
Gupta SK, Sundararaman V (1990) Biological response of earthwormPheretima posthuma to inorganic cadmium. Indian J Exp Biol 28:71–73
Hansch C, Leo A, Unger SH, Kim KH, Nikaitani D, Lien EJ (1973) Armatic substituents for struture-activity correlations. J Med Chem 16:1207–1216
Horobin RW (1981) Structure-staining relationships in histochemistry and biological staining III. Some comments on the intentional and artifactual staining of lipids. Acta Histochem [Suppl Band XXIV]:237–246
Horobin RW, Flemming L (1980) Structure-staining relationships in histochemistry and biological staining II. Mechanistic and practical aspects of the staining of elastic fibres. J Microsc 119:357–372
Itoh F, Shiraki M (1949) Patho-physical study of diffuse endocrinic system. V. Histochemical detection of some inorganic salts (II). Trans Jpn Pathol Soc 38:113–114
Mager M, McNary F, Lionetti F (1953) The histochemical detection of zinc. J Histochem Cytochem 1:493–504
Okamoto K, Hashimoto M (1944) Histochemical test method of zinc. Jpn J Constit Med 13:83–88
Parizek J, Zahor Z (1956) Effect of cadmium salts on testicular tissue. Nature 156:751–752
Perrin DD (1979) Stability constant of metal-ion complexes, part B. Organic ligands. Pergamon Press, Oxford
Prento P (1991) Selective, quantitative detection of cadmium and/or zinc by use of BTAN (2-aminobenzothiazolylazo-naphthol). Histochemistry 95:495–501
Shibata S, Furukawa M, Nakashima R (1976) Syntheses of azo dyes containing 4,5-diphenylimidazole and their evaluation as analytical reagents. Anal Chim Acta 81:131–141
Sumi Y (1980) Histochemical staining of cadmium with thiazolylazophenol derivatives. Histochemistry 67:1–6
Sumi Y, Miyazaki K, Tanaka A, Muraki T, Suzuki T (1979) Differential staining of cadmium and zinc by thiazolylazophenols. Acta Histochem Cytochem 12:460–464
Sumi Y, Muraki T, Suzuki T (1980) Histochemical staining of cadmium with benzothiazolylazophenol derivatives. Histochemistry 68:231–236
Sumi Y, Muraki T, Suzuki T (1982) Histochemical staining of cadmium with benzothiazolylazonaphthol derivatives. Histochemistry 73:481–486
Sumi Y, Muraki T, Suzuki T (1983a) The choice of masking agent in the histochemical staining of metals. Histochem J 15:231–238
Sumi Y, Muraki T, Suzuki T (1983b) A highly sensitive chelator for metal staining, bromopyridylazo-diethylaminophenol. Stain Technol 58:325–328
Sumi Y, Koyama Y, Muraki T, Suzuki T (1984a) The staining properties of thiazolylazoalkylaminophenol derivatives in histochemical staining of cadmium. Basic Appl Histochem 28: 137–142
Sumi Y, Suzuki T, Yamamura M, Hatakeyama S, Sugaya Y, Suzuki KT (1984b) Histochemical staining of cadmium taken up by the midge larva,Chironomus yoshimatsui (Deptera, chironomidae). Comp Biochem Physiol 79A:353–357
Sumi Y, Koyama Y, Muraki T, Suzuki T (1986) The staining properties of halo- and alkyl-thiazolylazophenol derivatives in hitochemical staining of cadmium. Basic Appl Histochem 30: 87–394
Ueno K, Sakaguchi T (1967) Metal chelates, vol. 3, Nakoudou, Tokyo, pp 15–22
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Sumi, Y., Itoh, M.T., Muraki, T. et al. Histochemical staining of cadmium with 2-(8-quinolylazo)-4,5-diphenylimidazole. Histochem Cell Biol 106, 223–227 (1996). https://doi.org/10.1007/BF02484404
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DOI: https://doi.org/10.1007/BF02484404