Summary
A method for electron microscopic demonstration of lipase has heen developed by means of lead precipitation technique, modifying the original technique for light microscopy by Gomori. Care was taken to reduce the concentration of lead nitrate solution for substitution in order to prevent both the non-specific lead deposition and the diffusion.
Applying the method to the pancreatic acinar cells of mice, specific activity was observed in rough surfaced endoplasmic reticulum, Golgi apparatus, zymogen granules and secretory canaliculi.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abe, M., S. P. Kramer, and A. M. Seligman: The histochemical demonstration of pancreatic like lipase and comparison with the distibution of esterase. J. Histochem. Cytochem. 12, 364–383 (1964).
Adams, C. W. M., Y. H. Abdulia, o. B. Bayliss, and R. O. Weller: Histochemical detection of triglyceride esters with specific lipases and a calcium-lead sulphide technique. J. Histochem. Cytochem. 14, 385–395 (1966).
Caro, C. G.: Electron microscopic radioautography of thin sections: The Golgi zone as a site of protein concentration in pancreatic acinar cells. J. biophys. biochem. Cytol. 10, 37–45 (1961).
Caro, L. G., and G. E. Palade: Protein synthesis, storage, and discharge in the pancreatic exocrine cell. An autoradiographic study. J. Cell Biol. 20, 473–495 (1964).
Gomori, G.: The microtechnical demonstration of sites of lipase activity. Proc. Soc. exp. Biol. (N. Y.) 58, 362–363 (1945).
—: Histochemical differentiation between esterases. Proc. Soc. exp. Biol. (N. Y.) 67, 4–6 (1948).
—: Histochemical localization of true lipase. Proc. Soc. exp. Biol. (N. Y.) 72, 697–700 (1949).
Krus, S., Z. A. Andrade, and T. Barka: Histochemical demonstration of specific phosphatases of the liver preserved in hypertonic sucrose solution. J. Histochem. Cytochem. 9, 487–490 (1961).
Kurata, Y., and M. Hoso: A new histochemical method for the demonstration of lipase [in Japanese]. Medicine and Biology (Tokyo) 18, 103–105 (1951).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Martin, B. F.: The histochemical test for lipase. Nature (Lond.) 172, 1048–1049 (1953).
McManus, J. F. A., and R. W. Mowry: Staining methods, histologic and histochemical, p. 169–170. New York: P. B. Hoeber, Inc. 1960.
Mizutani, A.: Fine structural demonstration of alkaline phosphatase activity [in Japanese with English summary]. Kumamoto med. J. 41, 57–70 (1967).
—, and R. J. Barrnett: Fine structural demonstration of phosphatase activity at pH 9. Nature (Lond.) 206, 1001–1003 (1965).
Mölbert, E. R. G., F. Duspiva, u. O. H. Deimling: Die histochemische Lokalisation der Phosphatase in der Tubulusepithelzelle der Mäuseniere im elektronenmikroskopischen Bild. Histochemie 2, 5–22 (1960).
Nachlas, M. M., and A. M. Seligman: Evidence for the specificity of esterase and lipase by the use of three chromogenic substrates. J. biol. Chem. 181, 343–355 (1949).
Ogawa, K., Y. Shinonaga, and T. Suzuki: Metallophilia of the striated border of the rat jejunal epithelial cells. Acta anat. nippon. 37, 134–139 (1962).
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Richterich, R.: Zur Technik der histochemischen Esterasenachweises. Acta anat. (Basel) 14, 263–296 (1952).
Sabatini, D., K. Bensch, and R. J. Barrnett: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963).
Sneath, P. H. A.: Histochemical demonstration of lipase. Nature (Lond.) 166, 699–700 (1950).
Takeuchi, T., M. Furuta, and K. Yoshimura: Supplement to histochemical demonstration of tissue lipase [in Japanese]. Tokyo med. J. 70, 319–321 (1953).
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958).
Wetzel, B. K., S. S. Spicer, and R. G. Horn: Fine structural localization of acid and alkaline phosphatases in cells of rabbit blood and bone marrow. J. Histochem. Cytochem. 15, 311–334 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murata, F., Yokota, S. & Nagata, T. Electron microscopic demonstration of lipase in the pancreatic acinar cells of mice. Histochemie 13, 215–222 (1968). https://doi.org/10.1007/BF00303755
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00303755