Summary
Based on former experiments dealing with a quantitative assay of acid phosphatase (acP) activity in histological sections by means of a laser microanalysis. The authors present results of investigations concerning optimal conditions needed for this purpose. The investigations were performed on rat liver sections. The realtion between incubation time and depth of acP reaction appearing in tissue blocks was investigated along with the dependence of photometric readings on thickness of histological sections and on incubation times. From results of experiments it appears that optimal conditions for quantitative determination of acP activity using the proposed laser method are provided by sections 30 to 50 μ in thickness which have been incubated in the substrate containing medium for 45 min. Sections thinner than 30 μ are not recommended for quantitative assay with this method because of low accuracy of readings caused by too low amounts of reaction product present there.
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Kozik, M., Warchoł, J. & Arcimowicz, B. Preparation of histochemical sections for quantitative determination of acid phosphatase activity by means of laser microanalysis. Histochemie 26, 74–79 (1971). https://doi.org/10.1007/BF00307786
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DOI: https://doi.org/10.1007/BF00307786