Summary
Ferritin labeled antibody to mouse liver catalase was applied to ultracryotomic sections of mouse liver tissue. The ferritin particles indicating a catalase antigen were localized in microbodies, membran-ebound ribosomes, cisternae of endoplasmic reticulum, Golgi vacuoles and vesicles, and cytoplasmic matrix of liver cell. Infrequently, the ferritin particles were observed in a few lysosomes of the hepatic cell and the stellate cell of Kupffer and in perichromatin of the hepatic cell nucleus. To the contray, mitochondria, Golgi lamellae, plasma membranes, and most of nuclei and lysosomes were not stained by ferritin conjugate. The results were discussed with regard to enzymecytochemical and biochemical results described by several investigators.
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Yokota, S., Nagata, T. Ultrastructural localization of catalase on ultracryotomic sections of mouse liver by ferritin-conjugated antibody technique. Histochemistry 40, 165–174 (1974). https://doi.org/10.1007/BF00495964
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DOI: https://doi.org/10.1007/BF00495964